嗜热微生物促进剩余污泥减量降解工艺优化

Thermophilic microorganisms promote process optimization of residue sludge reduction

以剩余污泥减量为目标,以淀粉酶和蛋白酶的活性为指标,考察了不同温度、转速及接种量下嗜热脂肪地芽孢杆菌的产酶特性.同时在最适条件下,将嗜热脂肪地芽孢杆菌接种入剩余污泥中进行降解减量,测定剩余污泥TSS、VSS、SCOD、氨氮、蛋白质的含量变化.结果表明,在55℃、120rpm、接种量12%的培养条件下,蛋白酶和淀粉酶活性最高,为5.64U/mL和0.051U/mL.在此培养条件下,接种嗜热脂肪地芽孢杆菌的剩余污泥的TSS溶解率达到了65.71%,较之对照的TSS溶解率高15.86%,而VSS的溶解率达到了58.18%,较对照要高16.51%;接种嗜热脂肪地芽孢杆菌处理的剩余污泥的SCOD的含量达到了3073mg/L,利于后续的厌氧生化处理;接种后污泥蛋白质含量变化呈现动态平衡,氨氮含量呈先升高后降低趋势,以上这些表明嗜热脂肪地芽孢杆菌的加入可促进剩余污泥的降解.

Based on the reduction of residual sludge and the activity of amylase and protease,the enzyme production characteristics of Bacillus stearo-thermo-philus under different temperatures,rotation speeds and inoculum quantities were studied.At the same time,under the optimum conditions,Bacillus stearo-thermo-philus was inoculated into the residual sludge and degraded to measure the change of TSS,VSS,SCOD,ammonia nitrogen and protein content in the residual sludge.The results showed that the protease and amylase activities were the highest at 55 ℃,120 rpm and 12% inoculum quantity,which was 5.64 U/mL and 0.051 U/mL.Under this culture condition,the TSS solubility rate of sludge inoculated with Bacillus stearo thermo philus reached 65.71%,which was 15.86% higher than the control,and the solubility rate of VSS reached 58.18%,16.51% higher than the control;The content of SCOD in the sludge inoculated with Bacillus stearo thermo philus reached 3 073 mg/L,which was beneficial to subsequent anaerobic biochemical treatment;The change of protein content in sludge after inoculation showed dynamic equilibrium,and the content of ammonia nitrogen increased first and then decreased,which indicated that the addition of Bacillus stearo thermo philus could promote the degradation of residual sludge.