摘要
为了获得适用于工业化生产的春雷霉素高产菌株,从陕西渭南某生物农药厂发酵生产车间不同发酵罐批中分离筛选到5株春雷霉素生产性能优良的小金色链霉菌,以这些菌株为出发菌株,利用递归原生质体融合法,获得了3株遗传稳定的高产春雷霉素重组菌株,其中1株重组菌株K2018SF5-06的春雷霉素产量达到了19.6g/L.经30吨罐生产验证,平均发酵效价比原始出发菌株K2010S17-182春雷霉素产量提高了37.1%.采用自然分离筛选结合递归原生质体融合育种技术能有效提高目标代谢产物的产量,产生巨大经济效益.
In order to obtain the high-yield strain suitable for industrial production of Kasugamycin,five strains of Streptomyces fuliginea with excellent performance for producing Kasugamycin were isolated from different fermenter batches in a fermentation company producing agricultural antibiotics in Shaanxi Province.Three genetically stable recombinant strains for high-yield of Kasugamycin were successfully selected by recursive protoplast fusion.The yield of one of the recombinant strains K2018S F5-06 reached 19.6 g/L.After 30 tons of pot production verification,the average fermentation potency increased by 37.1% compared with the original starting strain K2010 S 17-182.It indicates that the natural separation and screening combined with recursive protoplast fusion technology can effectively increase the yield of target metabolites and obtain huge economic benefits.
作者
田露
王亮
闵建红
祁晨娟
张志敏
龚国利
TIAN Lu;WANG Liang;MIN Jian-hong;QI Chen-juan;ZHANG Zhi-min;GONG Guo-li(School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi′an 710021, China;Shaanxi Microbe Biotechnology Co., Ltd., Weinan 714000, China;Shaanxi Institute of Microbiology, Xi′an 710043, China)
出处
《陕西科技大学学报》
CAS
2019年第4期52-57,共6页
Journal of Shaanxi University of Science & Technology
基金
陕西省科技厅重点研发计划项目(2018NY-104,2016NY-116)
陕西省教育厅服务地方专项科研计划项目(18JC004)
陕西省科学院科研计划项目(2016K-16)
关键词
春雷霉素
递归原生质体融合
小金色链霉菌
工业发酵
Kasugamycin
recursive protoplast fusion
Streptomyces faecalis
industrial fermentation