结肠腺瘤及腺癌间质FAP-α的表达

Expression of FAP-α in adenoma and adenocarcinoma stroma of colon

目的:通过观察结肠腺癌及腺瘤间质成纤维细胞激活蛋白(fibroblast activation protein-α,FAP-α)的表达情况,探讨结肠肿瘤间质在肿瘤发生发展中的作用,及其在诊断治疗及预后判断中的意义。方法:收集结肠腺瘤60例,结肠腺癌60例,其中p53阴性腺瘤组30例,p53阳性腺瘤组30例,p53阴性腺癌组30例,p53阳性腺癌组30例,用免疫组织化学检测肿瘤间质FAP-α的表达情况及肿瘤成分Ki-67的表达情况,用Image Pro Plus 6.0软件进行半定量分析,SPSS软件进行统计学分析。结果:间质FAP-α表达从弱到强依次为p53阴性结肠腺瘤、p53阳性结肠腺瘤、p53阴性结肠腺癌、p53阳性结肠腺癌(P<0.05);Ki-67值从低到高依次为p53阴性结肠腺瘤、p53阳性结肠腺瘤、p53阴性结肠腺癌、p53阳性结肠腺癌(P<0.05);p53阴性结肠腺癌与p53阳性结肠腺癌组FAP-α的表达与Ki-67呈正相关(P<0.05),p53阴性与p53阳性结肠腺瘤组FAP-的表达与Ki-67无明显相关性(P>0.05)。结论:结肠腺瘤与腺癌间质的FAP-α表达水平与肿瘤的生物学行为有关,进一步研究肿瘤间质对肿瘤的诊断及治疗有潜在价值。

Objective: To observe the expression of fibroblast activation protein-α(FAP-α) in colon adenocarcinoma and adenoma, and to discuss the role of colon stroma in tumor progression and its significance in diagnosis, treatment, and prognosis judgement. Methods: Colon adenoma and colon adenocarcinoma were collected in 120 cases, including 30 cases of p53 negative adenoma, 30 cases of p53 positive adenoma, 30 cases of p53 negative adenocarcinoma and 30 cases of p53 positive adenocarcinoma. Immunohistochemistry was used to detect the expression of FAP-α in tumor stroma and Ki-67 in tumor cells. Semi-quantitative analysis was performed with Image Pro Plus 6.0 software. SPSS software was used for statistical analysis. Results: The expression intensities of FAP-α in the stroma of the four groups from low to high were p53 negative colon adenoma, p53 positive colon adenoma, p53 negative colon adenocarcinoma and p53 positive colon adenocarcinoma (P<0.05). Ki- 67 values from low to high were p53 negative colon adenoma, p53 positive colon adenoma, p53 negative colon adenocarcinoma and p53 positive colon adenocarcinoma (P<0.05). The expression intensities of FAP-α in p53 negative and p53 positive colon adenocarcinomas were positively correlated with the Ki-67 value (P<0.05). The expression intensities of FAP-α in p53 negative colon adenoma and p53 positive colon adenoma were not significantly correlated with the Ki-67 value (P>0.05). Conclusion: The expression intensities of FAP-α in the stroma of colon adenoma and adenocarcinoma are related to the biological behavior of the tumors. Further study of the stroma of colon tumors has potential value for diagnosis and treatment.