潮霉素和草铵膦对4种草莓资源的选择效果分析

Evaluation of the selection effectiveness of hygromycin and glufosinate-ammonium for 4 strawberry germplasms

以‘红颜’‘申阳’‘章姬’‘Yellow Wonder’的离体叶片、叶柄、根为试验材料,对潮霉素(Hyg)敏感性进行研究;并以上述4种草莓资源的离体叶片为材料,探究其对草铵膦有效成分PPT的敏感性。结果表明:野生草莓‘Yellow Wonder’对两种筛选剂最为敏感。除‘申阳’草莓(叶片比根敏感)外,其他3种草莓资源均为根对Hyg较其叶片敏感。结合愈伤诱导受抑制情况,认为‘红颜’‘申阳’‘章姬’‘Yellow Wonder’叶片Hyg最佳选择压分别为4 mg/L、6 mg/L、6 mg/L、4 mg/L;PPT最佳选择压分别为0.8 mg/L、0.8 mg/L、1.0 mg/L、0.6 mg/L。

To explore the appropriate selection pressure for strawberry transformation,the sensitivity of different organs to hygromycin(Hyg)was studied using the leaf blades,petioles,and roots from 4 germplasms including cultivars‘Benihoppe’,‘Shen Yang’,‘Akihime’strawberry,and woodland strawberry‘Yellow Wonder’.Meanwhile,the sensitivity of leaf blades to Glufosinate-ammonium(PPT)was also investigated.The results showed that among the four strawberry germplasms used,the woodland strawberry‘Yellow Wonder’was most sensitive to both two selection agents.As compared to other organs used,roots from all germplasms except for cv.‘Shen Yang’were most sensitive to Hyg.But in cv.‘Shen Yang’,the leaf blade was more sensitive to Hyg than the root and petiole.Considering the callus induction from leaves under selection,it was recommended that the selective pressures of Hyg for‘Benihoppe’‘Shen Yang’‘Akihime’and‘Yellow Wonder’were 4 mg/L,6 mg/L,6 mg/L,and 4 mg/L,respectively.Furthermore,the suggested selection concentrations of PPT for leaves from‘Benihoppe’‘Shen Yang’‘Akihime’and‘Yellow Wonder’were 0.8 mg/L,0.8 mg/L,1.0 mg/L,and 0.6 mg/L,respectively.