矾根‘紫色宫殿’离体培养与快繁技术研究

Studies on in vitro culture and rapid propagation techniques of Heuchera ‘Palace Purple’

以矾根‘紫色宫殿’幼苗茎基部组织为外植体,开展丛生芽诱导、增殖和试管苗生根及移栽技术研究。结果表明:丛生芽诱导的适宜培养基是MS+6-BA 2.0 mg/L+NAA 0.1 mg/L,丛生芽诱导率达95.83%;丛生芽增殖的适宜培养基是MS+6-BA 0.2 mg/L+KT 1.0 mg/L+IAA 0.2 mg/L,增殖率达6.15;试管苗生根的最适培养基是1 2 MS+NAA 0.2 mg/L,生根率达100%。试管苗移栽的最适时期为4月上旬,并以塑料罩保湿为宜,成活率达96.67%,且植株生长良好。

Using the stem base tissue of Heuchera‘Palace Purple’seedlings as explants,the technique system of tissue culture were established,including cluster buds induction,multiplication culture,rooting culture and transplantation.Results showed that,the optimum medium of cluster buds induction was MS+6-BA 2.0 mg/L+NAA 0.1 mg/L,induction rate was 95.83%.The optimum medium of multiplication culture was MS+6-BA 0.2 mg/L+KT 1.0 mg/L+IAA 0.2 mg/L,the coefficient of multiplication was 6.15.The most suitable medium of rooting culture was 1 2 MS+NAA 0.2 mg/L,the rooting rate reached 100%.The fittest period of transplanting was the early of April,and after acclimatization and transplants,the transition cultivate survival rate of tube seedlings reached 96.67%by complement moisture using plastic caps in time,and they grew well.