不同稀释液中添加十二烷基硫酸钠对吐鲁番黑羊精液冷冻保存的影响

Effects of Different Diluents Supplement with Sodium Dodecylsulfate on Cryopreservation of Turpan Black Sheep Semen

为改善吐鲁番黑羊精液的冷冻保存效果,提高精液冻后的精子活率,本实验在3种精液冷冻稀释液中添加不同浓度(0、1%、2%)的十二烷基硫酸钠(SDS),TrⅠs-葡萄糖-柠檬酸钠为Ⅰ液,蔗糖-葡糖糖-柠檬酸钠为Ⅱ液,OptⅠdyl稀释液做对照为Ⅲ液。熏蒸冷冻后解冻,用精子分析系统(CASA)和流式细胞仪检测冷冻效果。结果表明:Ⅰ液1%SDS组精子活率最高(65.4%),与Ⅰ液2%SDS组精子活率没有差异,高于其他7组(P<0.05);Ⅲ液1%SDS组畸形率(9.4%)低于Ⅱ液0%SDS组、Ⅱ液2%SDS组和Ⅲ液2%SDS组(P<0.05);Ⅰ液1%SDS组直线运动速率(51.4μm/s)高于Ⅰ液0%SDS组、Ⅱ液2%SDS组和Ⅲ液0%SDS组(P<0.05);Ⅰ液1%SDS组和Ⅲ液1%SDS组的质膜完整率高于Ⅰ液2%SDS组和Ⅱ液1%SDS组(P<0.05),且Ⅰ液1%SDS组最高;Ⅰ液1%SDS组的顶体完整活精子比率高于Ⅱ液1%SDS组(P<0.05),各组的活精子顶体完整率无显著差异;Ⅰ液1%SDS组高线粒体膜电位比率(43.0%)高于Ⅱ液1%SDS组(P<0.05)。因此,采用添加1%SDS的Tris-葡萄糖-柠檬酸钠稀释液配方显著提高了吐鲁番黑羊精液冷冻保存效果。

In order to improve the cryopreservation effect of the Turpan black sheep sperm and improve sperm motility after semen freezing,in this experiment,different concentrations(0,1%,2%)of sodium dodecyl sulfate(SDS),were added to the three sperm frozen dilutions.Tris-Glucose-sodium citrate is liquid I,sucrose-glucose-sodium citrate is liquidⅡ,and Optidyl diluent is used as a control liquid.After fumigation and freezing,they were thawed,and the sperm analysis system(CASA)and flow cytometry were used to detect the freezing effect.The results showed that the sperm viability rate of the 1%SDS group in liquid I was the highest(65.4%),with no difference from that in the 2%SDS group of fluidⅠ,and significantly higher than that in the 7 groups(P<0.05);the Deformity rate of the 1%SDS group in liquidⅢwas the lowest(9.4%),significantly lower thanⅡliquid 0%SDS group,Ⅱliquid 2%SDS group andⅢliquid 2%SDS group(P<0.05);the linear motion rate of the 1%SDS group(51.4μm/s)in liquid I was the highest,significantly higher than I solution 0%SDS group,Ⅱliquid 2%SDS group andⅢliquid 0%SDS group(P<0.05).The plasma membrane integrity rate of theⅠliquid 1%SDS group and theⅢliquid 1%SDS group was significantly higher than that of theⅠliquid 2%SDS group and theⅡliquid 1%SDS group(P<0.05),and theⅠliquid 1%SDS group was the highest;The proportion of acrosome intact live sperm in theⅠliquid 1%SDS group was significantly higher than that in theⅡliquid 1%SDS group(P<0.05).The acrosome integrity rate of live sperm in each group was not significant difference;The proportion of high mitochondrial membrane potential in theⅠliquid 1%SDS group was the highest(43.0%),which was significantly higher than that in theⅡliquid 1%SDS group(P<0.05).Therefore,significantly improved Turpan Black Sheep semen cryopreservation result by using a Tris-glucose-sodium citrate dilution formulation with 1%SDS.