血浆microRNA-135a及microRNA-210在轻度认知损害患者中的检测价值

The detection of plasma microRNA-135a and microRNA-210 in the patient of mild cognitive impairment

目的探讨血浆microRNA-135a、microRNA-210在轻度认知损害患者中的诊断价值。方法收集于我院就诊的轻度认知损害患者及健康体检者的血液标本共80例,其中AMC组28例,VD-MCI28例,AD-MCI24例,采用RT-qPCR的方法进行血浆miRNA检测。结果VD-MCI组血浆microRNA-135a为(4.17×10^-7±8.35×10^-8);AD-MCI组血浆microRNA-135a为(2.83×10^-7±1.72×10^-7);AMC组血浆microRNA-135a为(9.87×10^-7±5.66×10^-7)。3组患者组间比较存在显著差异(F=30.76,P=0.00,P<0.05)。VD-MCI组血浆microRNA-210为(4.27×10^-5±1.09×10^-5);AD-MCI组血浆microRNA-210为(6.96×10^-5±2.48×10^-5);AMC组血浆microRNA-210(1.30×10^-5±7.07×10^-6)。3组患者组间比较存在显著差异(F=80.43,P=0.00,P<0.05)。3组患者MMSE评分与两个检测指标间无明显相关性(P>0.05)。结论血浆microRNA-135a及microRNA-210检测可辅助诊断轻度认知功能损害,对AD-MCI诊断价值更大。

Objective To evaluate the clinical value of plasma microRNA-135a,microRNA-210 in the detection of mild cognitive impairment.Methods The plasma samples of 80 subjects were collected from our hospital.There were 28 people with VD-MCI,24 people with AD-MCI and 28 age-matched control(AMC).RT-qPCR was applied to test the level of microRNA-135a and microRNA-210.Results The levels of microRNA-135a in plasma were(4.17×10^-7±8.35×10^-8),(2.83×10^-7±1.72×10^-7)and(9.87×10^-7±5.66×10^-7)in VD-MCI,AD-MCI and AMC.There were difference among three groups(F=30.76,P=0.00,P<0.05).The levels of microRNA-210 in plasma were(4.27×10^-5±1.09×10^-5),(6.96×10^-5±2.48×10^-5)and(1.30×10^-5±7.07×10^-6)in VD-MCI,AD-MCI and AMC.There were difference among three groups(F=80.43,P=0.00,P<0.05).There were no correlations between the MMSE score and the two biomarkers(P>0.05).Conclusions The plasma microRNA-135a and microRNA 210 were potential biomarkers of MCI,especially for AD-MCI.