射干苷和桃叶珊瑚苷抑制LPS诱导的人脂肪间充质干细胞炎性反应

Tectoridin and aucubin inhibit LPS-induced inflammatory response of human adipose mesenchymal stem cells

目的研究射干苷(tectoridin)、桃叶珊瑚苷(aucubin)对脂多糖(LPS)诱导后人脂肪间充质干细胞(hAMSCs)免疫调控作用的影响。方法通过CCK-8细胞增殖实验和细胞周期检测实验,筛选合适的药物作用浓度;筛选LPS作用浓度,建立炎性反应模型;用ELISA、RT-qPCR检测两者对LPS诱导后hAMSCs分泌促炎因子、抑炎因子的影响。结果50μg/mL射干苷和100μg/mL桃叶珊瑚苷能明显促进hAMSCs增殖(P<0.05,P<0.01);两者均促进LPS诱导后hAMSCs增殖(P<0.001);并且可抑制hAMSCs分泌IL-6、TNF-α、IFN-γ等促炎因子(P<0.05,P<0.01)。结论射干苷、桃叶珊瑚苷可显著抑制LPS诱导的hAMSCs炎性反应。

Objective To study the effects of tectoridin and aucubin on the immune regulation of human adipose mesenchymal stem cells(hAMSCs)induced by lipopolysaccharide(LPS).Methods CCK-8 cell proliferation test and cell cycle test were used to screen the appropriate concentration of drugs;LPS concentration was screened to establish an inflammatory response model;ELISA and RT-qPCR were used to detect the effects of both on the secretion of pro-inflammatory and anti-inflammatory factors by hAMSCs after LPS induction.Results Fiftyμg/mL tectoridin and 100μg/mL aucubin could significantly promote the proliferation of normal hAMSCs(P<0.05,P<0.01).Both of them promoted the proliferation of hAMSCs induced by LPS(P<0.001),and inhibited the secre-tion of IL-6,TNF-α,IFN-γ(P<0.05,P<0.01).Conclusions Tectoridin and aucubin may significantly inhibit LPS-induced hAMSCs inflammatory response.