摘要
目的探讨第10号染色体同源缺失性磷酸酶-张力蛋白(PTEN)对H2O2或丙泊酚诱导的大鼠胚胎心肌细胞系(H9c2)凋亡的影响。方法将H9c2心肌细胞分为对照组(正常培养细胞)、H2O2组(100μmol/L H2O2孵育24 h)、丙泊酚5、10和30μmol/L干预组,其中丙泊酚5、10和30μmol/L干预组细胞分别经H2O2处理24 h后加入不同浓度丙泊酚(5、10、30μmol/L)预处理1 h。MTT法检测细胞的活力;流式细胞计量术检测细胞凋亡率;RT-qPCR检测PTEN mRNA;检测过表达PTEN对丙泊酚处理的H2O2诱导的心肌细胞活力、凋亡、乳酸脱氢酶(LDH)和活性氧(ROS)的影响。结果与对照组相比,5和10μmol/L丙泊酚能提高H2O2诱导的心肌细胞活力、降低其凋亡率、抑制PTEN的表达量(P<0.05)。30μmol/L丙泊酚显著抑制细胞活力、促进细胞凋亡和PTEN的表达量(P<0.05);过表达PTEN可抑制丙泊酚对H2O2诱导的H9c2细胞的保护作用(P<0.05),促进LDH和ROS的表达量(P<0.05)。结论PTEN抑制丙泊酚对H2O2诱导的心肌细胞凋亡的保护作用。
Objective To investigate the effect of phosphatase and tensin homolog deletetion on chromosome 10(PTEN)on the apoptosis of H9c2 cardiomyocytes induced by H 2O 2.Methods H9c2 cardiomyocytes were divided into control group(normal cultured cells),H 2O 2 group(100μmol/L H 2O 2 treatment for 24 h),propofol(5,10,30μmol/L)group were treated with H 2O 2 for 24 h and then pretreated with propofol of different concentrations(5,10,30μmol/L)for 1 h.The viability of cells was detected by MTT assay and the apoptosis rate was measured by flow cytometry.The expression of PTEN was detected by RT-qPCR;The viability,apoptosis,lactate dehydrogenase(LDH),and reactive oxygen species(ROS)were analyzed for over-expression of PTEN.Results Compared with 0,5 and 10μmol/L propofol significantly increased the activity of cardiomyocytes induced by H 2O 2,decreased the rate of apoptosis,and inhibited the expression of PTEN(P<0.05);30μmol/L propofol significantly inhibited cell viability and promoted apoptosis and the expression of PTEN(P<0.05);over-expression of PTEN inhibited the protective effect of propofol against H 2O 2-induced H9c2 cells(P<0.05),and promote the expression of LDH as well as ROS(P<0.05).Conclusions PTEN inhibits the protective effect of propofol against H 2O 2-induced apoptosis of cardiomyocytes.
作者
刘宇
何雨
李耀
万永灵
LIU Yu;HE Yu;LI Yao;WAN Yong-ling(Sichuan Provincial People's Hospital,Chengdu 610000,China)
出处
《基础医学与临床》
CSCD
2019年第7期1025-1030,共6页
Basic and Clinical Medicine