结节性痒疹皮损中胸腺基质淋巴生成素及其受体表达的研究

Expression of Thymic Stromal Lymphopoietin and Its Receptor in Prurigo Nodularis

目的探讨结节性痒疹(Prurigo nodularis,PN)皮损中胸腺基质淋巴生成素(TSLP)及其受体的表达情况。方法留取PN患者皮损处、皮损周围及正常皮肤标本,通过实时荧光定量PCR和免疫组化染色方法,分别从转录水平和蛋白水平比较各组之间TSLP、TSLP异源受体双链胸腺基质淋巴生成素受体(TSLPR)和白细胞介素-7受体α(IL-7RA)的表达差异。结果PN皮损与皮损周围相比,皮损部位TSLP、TSLPR和IL-7RA的相对表达量均略高于皮损周围,但是二者之间的差异均无统计学意义(t=0.667,P=0.519;t=0.945,P=0.367;t=0.151,P=0.883)。PN皮损部位与正常皮肤相比,TSLP表达差异无统计学意义(t=0.810,P=0.429),TSLPR和IL-7RA表达则显著高于正常皮肤(t=3.57,P=0.0051;t=2.552,P=0.0287)。PN皮损周围和正常皮肤相比,TSLP表达差异无统计学意义(t=0.448,P=0.659),TSLPR和IL-7RA表达高于正常皮肤,但是差异无统计学意义(t=1.659,P=0.128;t=1.732,P=0.114)。免疫组化染色结果显示PN皮损表皮全层TSLP呈阳性反应,真皮内部分炎症细胞及真皮神经纤维TSLPR呈阳性反应,IL-7RA广泛表达于表皮全层和真皮内炎症细胞。结论TSLP的2个异源受体亚单位TSLPR和IL-7RA在PN患者皮损处表达显著升高,提示TSLP-TSLPR通路可能参与PN瘙痒的发生。

Objective In order to investigate the expression of thymic stromal lymphopoietin(TSLP)and its receptors in the lesions of prurigo nodularis(PN).Methods Lesional skin of PN,perilesional skin of PN and normal skin specimens were collected.The mRNA and protein levels of TSLP,TSLPR and IL-7RA were compared among the three groups by real-time fluorescence quantitative PCR and immunohistochemical staining respectively.Results The relative expressions of TSLP,TSLPR and IL-7RA in lesional skin were slightly higher than those in perilesional skin in PN patients,but there were no significant differences between two groups(t=0.667,P=0.519;t=0.945,P=0.367;t=0.151,P=0.883).Expression of TSLP showed no differences compared with normal skin(t=0.810,P=0.429),while expressions of TSLPR and IL-7RA were significantly higher than those in normal skin(t=3.57,P=0.0051;t=2.552,P=0.0287).There was no significant difference in TSLP expression between perilesional skin and normal skin(t=0.448,P=0.659).Expressions of TSLPR and IL-7RA were higher than normal skin,but the differences showed no statistically significant(t=1.659,P=0.128;t=1.732,P=0.114).The results of immunohistochemistry showed that TSLP was positive in the full-thickness epidermis of PN lesion.TSLPR was positive in some inflammatory cells in dermis and nerve fibers.IL-7RA was widely expressed in the whole epidermis and dermal inflammatory cells.Conclusion Expressions of TSLP receptors,TSLPR and IL-7RA,increased significantly in lesions of PN patients,which suggested that TSLP-TSLPR pathway may be involved in the pathogenesis of itching in PN.