摘要
A method for quantitative determination of fish sperm deoxyribonucleic acid(fsDNA)was developed by using titanium dioxide(TiO2)as an adsorbent and near-infrared diffuse reflectance spectroscopy(NIRDRS).The selective enrichment of fsDNA was proved by comparing the adsorption efficiency of bovine serum albumin,tyrosine and tryptophan,and the low adsorption background of TiO2 was illustrated by comparing the spectra of four commonly-used inorganic adsorbents(alkaline aluminium oxide,neutral aluminium oxide,nano-hydroxyapatite and silica).The spectral feature of fsDNA can be clearly observed in the spectrum of the sample.Partial least squares(PLS)model was built for quantitative determination of fsDNA using 28 solutions,and 13 solutions with interferences were used for validation of the model.The results showed that the correlation coefficient(R)between the predicted and the reference concentration is 0.9727 and the recoveries of the validation samples are in the range of 98.2%-100.7%.
A method for quantitative determination of fish sperm deoxyribonucleic acid(fsDNA) was developed by using titanium dioxide(TiO2) as an adsorbent and near-infrared diffuse reflectance spectroscopy(NIRDRS). The selective enrichment of fsDNA was proved by comparing the adsorption efficiency of bovine serum albumin, tyrosine and tryptophan, and the low adsorption background of TiO2 was illustrated by comparing the spectra of four commonly-used inorganic adsorbents(alkaline aluminium oxide, neutral aluminium oxide, nano-hydroxyapatite and silica). The spectral feature of fsDNA can be clearly observed in the spectrum of the sample. Partial least squares(PLS) model was built for quantitative determination of fsDNA using 28 solutions, and 13 solutions with interferences were used for validation of the model. The results showed that the correlation coefficient(R) between the predicted and the reference concentration is 0.9727 and the recoveries of the validation samples are in the range of98.2%–100.7%
基金
supported by the National Natural Science Foundation of China(No.21775076)
the fundamental research funds for central universities(China)
