摘要
Two fluorescent“off-on”probes YYH1 and YYH2 were used for the detection of mitochondrial polarity and viscosity.Both probes have two emission bands:The peak of shorter wavelength was sensitive to the polarity,while that in longer wavelength region was sensitive to the viscosity,which enables us to detect medium polarity and viscosity simultaneously.The lg(Ishort/Ilong)of YYH1 exhibited linear relationship to the solvent polarity,while that of YYH2 was closely related to the solvent viscosity.Furthermore,both probes showed potential in bioimaging mitochondrial polarity or viscosity in living cells.
Two fluorescent "off-on" probes YYH1 and YYH2 were used for the detection of mitochondrial polarity and viscosity. Both probes have two emission bands:The peak of shorter wavelength was sensitive to the polarity, while that in longer wavelength region was sensitive to the viscosity, which enables us to detect medium polarity and viscosity simultaneously. The lg(Ishort/Ilong) of YYH1 exhibited linear relationship to the solvent polarity, while that of YYH2 was closely related to the solvent viscosity. Furthermore, both probes showed potential in bioimaging mitochondrial polarity or viscosity in living cells.
基金
the National Natural Science Foundation of China(No.21576085)for financial support
