摘要
Construction of multi-enzyme systems not only can help expand the understanding of physiological and pathological mechanisms,but also holds great promise in biosynthesis,biosensing and biomedicine.Recently,lots of strategies have been exploited to arrange multiple enzymes into a confined space for the construction of artificial multi-enzyme complexes,including liposomes encapsulating,polymersomes entrapping,chemical crosslinking and covalent conjugation.Although these strategies are promising,there are still several obstacles remaining which may impede the practical applications.For example,synthesis of these multi-enzyme complexes involves tedious preparations and multiple steps,leading to the loss of enzyme activity and further decreasing the catalytic efficiency.Therefore,a simple and efficient strategy to develop multi-enzyme complexes remains a great challenge.
