玉米PIN家族基因在雌花序发育过程中的表达分析

Expression Analysis of ZmPIN Family Genes in Female Inflorescence Development of Maize

生长素是一类重要的植物发育调控因子,其代谢和转运在植物的器官形成和分化中均发挥重要作用。植物特有的生长素外运载体PIN是一类膜蛋白,和生长素极性运输相关。本研究中,我们对玉米PIN基因家族的14个成员在雌花序发育过程中的表达模式进行了分析。除了未检测到ZmPIN9基因的表达外,其余13个基因根据表达模式可分为三类:第一类包括ZmPIN1a、ZmPIN1b、ZmPIN1d、ZmPIN5c和ZmPIN8五个基因,在SPM(小穗对原基)时期表达量最高;第二类包括ZmPIN1c、ZmPIN10b、ZmPINx和ZmPINy四个基因,在SM1(小穗原基早期)时期表达量最高;第三类包括ZmPIN2、ZmPIN5a、ZmPIN5b和ZmPIN10a四个基因,在F(花器官)时期表达量最高。为了进一步了解ZmPIN家族基因的表达模式,我们对这14个基因在不同组织器官中的表达模式进行了分析,依然未检测到ZmPIN9基因的表达,可能是其表达量极低或在特定部位或特定时期表达;ZmPIN5a基因在叶中的表达量最高;其余12个ZmPIN基因均在雄花序中高表达。这些结果说明ZmPIN基因在玉米生殖器官尤其是雌花序的发育过程中起重要作用。

Auxin is an important plant development regulator,and its metabolism and transport play important roles in organ formation and differentiation.The plant specific auxin transport carrier PIN is a membrane protein,which is associated with the polar transport of auxin.In this paper,we analyzed the expression patterns of 14 ZmPIN genes in female inflorescence development.In addition to undetected the expression of ZmPIN9,the other 13 genes could be divided into three classes according to the expression pattern.The first class included five genes as ZmPIN1a,ZmPIN1b,ZmPIN1d,ZmPIN5c and ZmPIN8,which had the highest expression level in SPM phase.The second class included four genes as ZmPIN1c,ZmPIN10b,ZmPINx and ZmPINy,which had the highest expression level in SM1 phase.The third class included four genes as ZmPIN2,ZmPIN5a,ZmPIN5b and ZmPIN10a,which had the highest expression in F phase.To further understand the expression patterns of ZmPIN family genes,we also analyzed the expression patterns of these 14 genes in different tissues and organs.The expression of ZmPIN9 gene was still not detected,which might be due to its extremely low expression level or its expression at a specific location or a specific period.The expression level of ZmPIN5a gene in leaves was the highest.The remaining 12 ZmPIN genes were highly expressed in male inflorescence.These results suggested that ZmPINs should play an important role in the development of reproductive organs,especially in female inflorescence development.The results of this study would provide important information about the function of ZmPIN family genes in the female inflorescence development of maize.