无透镜显微技术

Lens-free Microscopy

无透镜显微成像(lens-free microscopy)是一种在不借助透镜的情况下进行成像的技术。它基于Gabor同轴全息原理,利用面阵探测器采集原始全息图,随后通过数字图像处理技术重建样本,从而实现数字显微成像。像素超分辨技术缩小了等效像素,提供更多细节信息使得再现像的分辨率得以直接提升,而且多种相位恢复手段通过去除孪生像也达到了间接提高分辨率的目的,尤其是对密集样本。无透镜显微成像技术突破了传统光学显微镜由透镜带来的空间带宽积的限制,实现了大视野范围下的高分辨率成像,因此,这一技术能够提供大视场下的临床样本快速诊断和准确检测。另外,新兴的算法和硬件都在不断地加快数据采集和计算速度,扩展了其在高速运动样本和纳米尺度样本上的应用。最近无透镜技术和其配套硬件设备发展方向趋向于硬件紧凑、算法密集、实时、三维、彩色、高分辨率的便携式分立器件或配件。

Lens-free microscopic technology utilizes Gabor-based in-line holographic optical path to capture the interference images generated by illuminating lights through the micro-samples, with the help of an area array detector but without any imaging lenses. It is a digital microscopic imaging technology which uses a digital-picture-processing method to reconstruct image for the sake of acquiring information from these micro-samples. The shrinkage of equivalent pixel elements achieved by pixel super-resolution gives more high-frequency information and, further, it promotes the resolution of reconstruction directly. Meanwhile, multiple phase recovery arithmetic tools make the same contributions to resolution indirectly by eliminating the twin-images, especially to the dense samples. Moreover, such technology breaks the limitation in spatial bandwidth product, comparing to the classical optics microscopes, which leads to the high-resolution imaging in a larger field of view (FOV). Therefore, it can provide powerful supports for rapidly diagnosing samples with a big field of view in clinical application and examination in the case of resource shortage. Additionally, the booming algorithm with optimizing hardwares renders impetus in data-sampling and computing effects, expanding the applications on specimens moving in high speed and on a nanometer scale. The developing tendency of lens-free technology and its matched hard devices are to become compacted both arithmetically and volumetrically. Ultimately, the real-time, three dimensional, colorfully-imaging, portable and separated devices with high-resolution can be manufactured.