铺地锦竹草水提取物主要成分及抗氧化活性研究

Composition and Antioxidization Effect of Callisia repens Extracts

以市售铺地锦竹草为材料制备其水提取物,采用分光光度法测定总黄酮、总花色苷、总糖等主要活性成分含量,采用ICP-MS质谱仪法测定提取物中18种金属元素的组成和含量,最后采用分光光度法研究提取物的体外抗氧化活性。结果显示,铺地锦竹草提取物中黄酮含量为2.04%,总蛋白含量1.83%,总糖含量55%,总花色苷含量7.2%,Ca、Mn、Mg等有益微量元素含量较高,Pb、Hg、Ag、Co、Ge等有害重金属微量检出或未检出。提取物清除DPPH·的IC50达0.265mg·mL^-1,清除·OH自由基IC50为1.16mg·mL^-1,1mg提取物的总还原力与39μgVc相当,对亚铁离子未表现出有规律的螯合力。以上说明铺地锦竹草提取物中天然活性成分含量较高,有害重金属极微,具有良好的抗氧化能力,其抗氧化活性是由多种活性因子通过协同作用机制起作用的,具有开发为功能性食品的良好潜力。

To study the main active ingredients and antioxidant activity in vitro of extracts from Callisia repens,the contents of main active ingredients such as total flavonoids,total anthocyanin and total sugar in the extracts were studied by spectrophotometry.The composition and content of various metals were determined by ICP-MS mass spectrometry.The antioxidant activity of the extract was evaluated by spectrophotometry.Results showed that the content of flavonoids,the total protein,the total sugar and the total anthocyanin in C.repens extract powder were 2.04%,1.83%,55.2%and 7.2%,respectively.Ca,Mn,Mg and other beneficial trace elements in C.repens extracts were higher,while Pb,Hg,Ag,Co,Ge and other harmful heavy metals were detected very tiny or not at all.The IC50 of C.repens extracts was 0.265 mg·mL^-1 for DPPH·,1.16 mg·mL^-1 for·OH,the total reducing power of 1 mg extracts was equal to 39μg Vc,and extracts showed no regular chelating power to ferrous ions.C.repens extract had good reducibility and antioxidant activity and its antioxidant activity was affected by a variety of active factors through a synergistic mechanism.