复方克林霉素脂质体凝胶的制备及体外透皮释药研究

Preparation and in vitrotransdermal diffusion characteristics of Compound Clindamycin Liposome Gel

目的制备复方克林霉素脂质体凝胶并考察其粒径分布、包封率及体外透皮特性。方法采用薄膜分散法制备复方克林霉素脂质体,利用透射电镜观察其形态,用激光纳米粒度仪测定其粒径大小及分布,用HPLC法测定其包封率。将脂质体进一步制成凝胶剂后,考察其体外透皮情况。结果复方克林霉素脂质体的粒径在240nm左右,分布均匀,平均包封率为51.24%;脂质体中的克林霉素能缓慢透过大鼠皮肤,缓释效果明显。结论该制剂制备工艺简单,性质稳定,药物包封率较高,定量测定方法简便、准确;药物透皮速率缓慢,释药稳定。

Objective To prepare Compound Clindamycin Liposome Gel and investigate its particle size distribution,encapsulation rate and in vitro transdermal properties.Methods Compound clindamycin liposomes were prepared by thin film dispersing method,their morphology was observed by radio microscope,particle size and distribution were determined by laser nanometer granulometer,and the encapsulation rate was determined by HPLC.After the liposomes were further prepared into gels,the skin penetration in vitro was investigated.Results The particle size of compound clindamycin liposomes was evenly distributed,and the particle size was about 240 nm,with an average encapsulation rate of 51.24%.Clindamycin in liposomes can pass through the skin of rats slowly and release slowly.Conclusion The preparation process is simple,the property is stable,the drug encapsulation rate is high,the quantitative determination method is simple and accurate.Drug penetration rate is slow and drug release is stable.