Ⅰ群禽腺病毒血清4型TaqMan探针实时荧光定量PCR检测方法的建立及应用

Establishment and Application of TaqMan Real-time PCR for Detection of Fowl Adenovirus Group Ⅰ Serotype 4

为储备禽腺病毒病疫情防控技术,应用DNAStar软件分析GenBank中Ⅰ群禽腺病毒血清4型(FAdV-4)全基因序列,选取Hexon基因的保守区域设计合成1对PCR引物和1条TaqMan探针,建立FAdV-4FQ-PCR检测方法,得到相应的标准曲线,并应用该方法对临床病例和人工感染动物进行FAdV-4核酸检测。结果表明,建立的FAdV-4FQ-PCR方法具有良好的敏感性、特异性、稳定性和临床应用性。

In order to reserve the technology for prevention and control of avian adenovirus disease,the whole gene sequences of groupⅠavian adenovirus serotype 4(FADV-4)were analyzed with the DNAStar software and the conserved sequence of Hexon gene was selected to synthesize a pair of PCR primers and a TaqMan probe.FAdV-4 FQ-PCR detection method and corresponding standard curve were established and used to detect the samples of clinical cases and artificially infected cases.The results showed that the FAdV-4 FQ-PCR method had good sensitivity,specificity,stability and clinical applicability.The establishment of this method will promote the research in rapid detection and pathogenes of Fowl Adenovirus groupⅠSerotype 4.