核苷酸结合寡聚化结构域样受体蛋白3与非酒精性脂肪肝的相关性分析

Analysis of correlation between NLR family pyrin domain containing 3 and nonalcoholic fatty liver disease

目的探讨核苷酸结合寡聚化结构域样受体蛋白3(NLR family pyrin domain containing 3,NLRP3)与非酒精性脂肪肝(nonalcoholic fatty liver disease,NAFLD)的相关性。方法采用横断面调查研究,随机选取上海健康医学院附属周浦医院2017年1月体检人员84名,通过肝脏超声明确有无NAFLD,分为NAFLD组(27例)及正常对照组(57例),分析两组研究对象一般资料及肝功能、血脂、血糖、肌酐、尿酸等生化指标;运用ELISA双抗体夹心法检测外周血NLRP3水平。通过Logistic回归分析NLRP3与NAFLD的关系。结果NAFLD患病率为32.1%(27/84)。NAFLD组患者血清胆固醇、甘油三酯、低密度脂蛋白胆固醇、空腹血糖、丙氨酸氨基转移酶、肌酐及尿酸水平均高于正常对照组(P均<0.01),血清高密度脂蛋白胆固醇水平则低于正常对照组(P=0.023);NAFLD组血清NLRP3浓度为(5.1±1.8)μg/L,正常对照组为(3.9±1.4)μg/L,两组比较差异有统计学意义(t=6.221,P=0.016)。Logistic回归分析显示,NLRP3是NAFLD发生的风险因素(OR=1.537,P=0.021)。结论NLRP3水平在NAFLD患者血清中上调,与NAFLD的发生相关。

Objective To explore the correlation between NLR family pyrin domain containing 3 (NLRP3) and nonalcoholic fatty liver disease (NALFD). Methods A cross-sectional study was conducted in this study.Eighty four cases of health examination personnel in Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital were included in the study randomly, and divided into NAFLD group(27cases) and normal control group(57cases) though liver ultrasound examination.Their general data (sex, height, weight), liver function, blood lipid, and blood glucose were analyzed.The level of NLRP3 in peripheral blood was analyzed by ELISA double antibody sandwich method.The relationship between NLRP3 and NAFLD was analyzed by Logistic regression. Results The prevalence of NAFLD was 32.1%(27/84). The levels of serum cholesterol, triglyceride, low density lipoprotein cholesterol, fasting blood sugar, alanine aminotransferase, creatinine and uric acid in NAFLD group were higher than those in normal control group (all P<0.01), while the level of serum high density lipoprotein cholesterol was lower than that in normal control group (P=0.023);the concentration of serum NLRP3 in NAFLD group was (5.1±1.8)μg/L, and that in normal control group was (3.9±1.4)μg/L.There was significant difference between the two groups (t=6.221, P=0.016). Logistic regression analysis showed that NLRP3 was a risk factor for NAFLD (OR=1.537, P=0.021). Conclusion The level of NLRP3 is up-regulated in the serum of NAFLD patients, which is related to the occurrence of NAFLD.