囊包形成初期旋毛虫感染小鼠脾脏分泌白细胞介素-22的淋巴细胞来源分析

Source analysis of lymphocytes secreting interleukin-22 from spleen of mice infected with Trichinella spiralis at the early encapsulated stage

目的探讨囊包形成初期旋毛虫感染的小鼠脾脏分泌白细胞介素(IL)-22的淋巴细胞来源。方法选择12只Balb/c小鼠,按体重采用随机数字表法分为感染组与对照组,每组6只。感染组小鼠经口感染旋毛虫肌幼虫300条/只,对照组给予等量生理盐水灌胃。于感染后第4周用流式细胞术分析两组小鼠脾脏淋巴细胞中分泌IL-22的细胞亚群。结果感染组小鼠脾脏淋巴细胞中产生IL-22的淋巴细胞比例相比于对照组明显升高[(0.88±0.25)%比(0.28±0.17)%,t =- 4.899,P<0.05];感染组小鼠脾脏淋巴细胞中CD3^+IL-22^+细胞比例与CD3-IL-22^+细胞比例相比未见明显改变[(0.29±0.17)%比(0.51±0.17)%,t =- 2.195,P > 0.05],感染组CD3-IL-22^+细胞比例与对照组相比,差异无统计学意义[(0.51±0.17)%比(0.44±0.22)%,t =- 0.600,P > 0.05],感染组CD3^+IL-22^+细胞比例相比于对照组显著升高[(0.29±0.17)%比(0.07±0.06)%,t =- 3.068,P<0.05];CD3^+淋巴细胞中,感染组小鼠CD4^+IL-22^+ T细胞比例和γδTCR^+IL-22^+ T细胞比例相比于对照组明显升高[(1.28±0.54)%比(0.16±0.07)%,(0.33±0.22)%比(0.02±0.00)%,t =- 4.997、- 3.342,P均< 0.05]。结论囊包形成初期感染旋毛虫的小鼠脾脏分泌IL-22的淋巴细胞比例增高,主要由分泌IL-22的CD3^+淋巴细胞(尤其是分泌IL-22的CD4^+ T细胞和γδT细胞)比例升高所致。

Objective To investigate the cell origin of interleukin (IL)-22-secreting cell of mice infected with Trichinella spiralis (T.spiralis) at the early encapsulated stage.Methods Twelve Balb/c mice were divided into the infected group and the control group according to body weight by random number table.The infected mice were intragastrically administrated with 300 muscle larvae of T.spiralis,and the control mice were given the same amount of normal saline.The IL-22-secreting cell subsets in mouse splenic lymphocytes were detected by flow cytometry at the fourth week after infection.Results The proportion of IL-22-secreting cells in splenic lymphocytes of T.spiralis infected mice was increased when compared with control group [(0.88 ± 0.25)% vs (0.28 ± 0.17)%,t=-4.899,P<0.05].There was no significant difference between the proportion of CD3^+IL-22^+ cells and CD3-IL-22^+ cells in the splenic lymphocytes of the infected group [(0.29 ± 0.17)% vs (0.51 ± 0.17)%,t=-2.195,P > 0.05],and the percentage of CD3-IL-22^+ cells were similar between the infected group and the control group [(0.51 ± 0.17)% vs (0.44 ± 0.22)%,t=-0.600,P > 0.05].The proportion of CD3^+IL-22^+ cells in the infected group was significantly higher than that in the control group [(0.29 ± 0.17)% vs (0.07 ± 0.06)%,t=-3.068,P<0.05],and the percentage of CD4^+IL-22^+ T cells and γδTCR^+IL-22^+ T cells were obviously increased in CD3^+ lymphocytes [(1.28 ± 0.54)% vs (0.16 ± 0.07)%,(0.33 ± 0.22)% vs (0.02 ± 0.00)%,t=-4.997,-3.342,P<0.05].Conclusions The proportion of IL-22-secreting splenic lymphocytes is increased in mice infected with T.spiralis at the early encapsulated stage.The rise is caused by increased numbers of IL-22-secreting CD3^+ lymphocytes,especially CD4^+ T cells and γδT cells.