摘要
A novel phosphoprotein separation material was developed, which is constructed by a magnetic mesoporous Fe3 O4@TiO2（Fe3 O4@mTiO2） microsphere and a 5-aminoisophthalic acid（AIPA） monolayer that provides additional binding sites toward phosphate groups. The results of characteristic experiments demonstrated that Fe3 O4@mTiO2-AIPA had good dispersability, high magnetic susceptibility, and satisfactory grafting ratio of AIPA, ascribed to the large specific surface area of the inorganic substrate. Taking advantages of these features, Fe3 O4@mTiO2-AIPA was successfully utilized to separate α-casein（a typical phosphoprotein） and bovine serum albumin（BSA, a typical non-phosphoprotein） from their mixtures（molar ratio = 1:2）. Through adjusting pH and polarity of solutions, the BSA and α-casein were respectively enriched in washing fraction and elution fraction. This result displays the good potential of Fe3 O4@mTiO2-AIPA for application in phosphoprotein enrichment.
A novel phosphoprotein separation material was developed, which is constructed by a magnetic mesoporous Fe3O4@TiO2 (Fe3O4@mTiO2) microsphere and a 5-aminoisophthalic acid (AIPA) monolayer that provides additional binding sites toward phosphate groups. The results of characteristic experiments demonstrated that Fe3O4@mTiO2-AIPA had good dispersability, high magnetic susceptibility, and satisfactory grafting ratio of AIPA, ascribed to the large specific surface area of the inorganic substrate. Taking advantages of these features, Fe3O4@mTiO2-AIPA was successfully utilized to separate a-casein (a typical phosphoprotein) and bovine serum albumin (BSA, a typical non-phosphoprotein) from their mixtures (molar ratio = 1:2). Through adjusting pH and polarity of solutions, the BSA and a-casein were respectively enriched in washing fraction and elution fraction. This result displays the good potential of Fe3O4@mTiO2-AIPA for application in phosphoprotein enrichment.
作者
TANG Qiuhan
LU Qi
QING Guangyan
汤秋菡;ZHAO Rui;陆琦;卿光焱(School of Chemistry,Chemical Engineering and LifeScience,Wuhan University of Technology,Wuhan430070,China;State Key Laboratory of Advanced Technology for Materials Synthesis and Processing,Wuhan University of Technology,Wuhan430070,China;Research &Development Center,Jushi Group Co.,Ud,Tongxiang314500,China)
基金
Funded by the National Natural Science Foundation of China(Nos.51473131,21275114,51533007 and 51521001)
the Major State Basic Research Development Program of China(973 Program)(No.2013CB933002)
Hubei Provincial Department of Education for Financial Assistance Through the “Chutian Scholar” Program
Hubei Provincial Natural Science Foundation of China(No.2014CFA039)
