低能量激光联合牙周基础治疗对种植体周围炎龈沟液中IL-8、b-FGF及IL-1β水平的影响

Effect of low le vel laser treatment combined with periodontal initial therapy on IL-8,b-FGF and IL-1β content in gingival crevicular fluid on peri-implantitis

目的研究低能量激光联合牙周基础治疗对种植体周围炎龈沟液中白细胞介素-8(IL-8)、碱性成纤维细胞生长因子(b-FGF)及白细胞介素-1β(IL-1β)水平的影响,评价该治疗方法的疗效.方法选择早期种植体周围炎患者38例(共50颗种植体),随机分为实验组和对照组,每组19例患者(25颗种植体).实验组采用低能量激光联合牙周基础治疗,对照组仅采用牙周基础治疗.分别于治疗前(基线)、治疗后4周和12周采集两组种植体龈沟液,酶联免疫吸附法测定其中IL-8、b-FGF及IL-1β水平,应用SPSS19.0软件对数据进行统计分析.结果治疗后4周和12周实验组与对照组龈沟液中的IL-8、b-FGF及IL-1β水平较基线均有改善,其差异均具有统计学意义(均P<0.01);同组内不同时间点进一步两两比较,龈沟液中的IL-8和b-FGF水平差异均具有统计学意义(均P<0.01);同组内治疗后4周与治疗后12周比较,IL-1β水平差异均无统计学意义(均P>0.05).治疗后4周实验组龈沟液中的IL-8及IL-1β水平均低于对照组,差异均具有统计学意义(均P<0.05);治疗后12周实验组龈沟液中的b-FGF水平高于对照组,差异具有统计学意义(P<0.05).结论低能量激光可影响种植体周围炎龈沟液中的细胞因子水平,其联合牙周基础治疗对种植体周围炎有治疗效果.

Objective To investigate the effect of low level laser treatment combined with periodontal therapy on interleukin-8 (IL-8),basic fibrobast growth factor (b-FGF) and interleukin-1β(IL-1β) level in gingival crevicular fluid on peri-implantitis,so as to evaluate the curative effect. Methods A total of 38 patients (50 implants) with early peri-implantitis were randomly divided into experimental group and control group,with 19 patients (25 implants) in each group. The patients in the experimental group were underwent level laser treatment combined with periodontal initial,and in the control group were only treated with periodontal initial. The implant gingival crevicular fluid was collected before the treatment (baseline),and 4 weeks and 12 weeks after the treatment. The levels of IL-8,b-FGF and IL-1β were determined by enzyme-linked immunosorbent assay (ELISA). The SPSS19.0 software was used for the statistically analysis. Results The levels of IL-8,b-FGF and IL-1β of the experimental group and the control group were both improved at 4 weeks and 12 weeks after treatment comparing with that at the baseline,and the differences were statistically significant (all P<0.01). The differences in IL-8 and b-FGF levels in the gingival crevicular fluid were statistically significant at different time points for each group (all P<0.01). For the two groups,the differences in IL-1β were not statistically significant at 4 weeks and 12 weeks after the treatment (all P>0.05). The levels of IL-8 and IL-1β of the experimental group were lower than those of the control group at 4 weeks after treatment,and the differences were statistically significant (all P<0.05). The level of b-FGF of the experimental group was higher than that of the control group at 12 weeks after the treatment,and the difference was statistically significant (P<0.05). Conclusions Low-energy laser can affect the level of cytokines in the gingival crevicular fluid on peri-implantitis. The combination of low level laser treatment and periodontal initial therapy is effective for treating peri-implantitis.