25%呋虫胺·嘧菌酯·种菌唑种子处理悬浮剂液相色谱分析方法

Analytical Method of Dinotefuran + Azoxystrobin + Ipconazole 25% FS by HPLC

本文采用高效液相色谱法,以乙睛和水为流动相进行梯度洗脱,使用ZORBAX80A Extend-C18不锈钢柱和二极管阵列检测器,在222nm波长下对混剂中呋虫胺、嘧菌酯和种菌唑进行分离和定量分析。结果表明,该方法测得呋虫胺、嘧菌酯和种菌唑的线性相关系数分别为1.0000、1.0000、0.9999;标准偏差分别为0.11、0.07、0.01;变异系数分别为0.59%、1.24%、1.38%;平均回收率分别为99.85%、100.25%、100.14%。

A method for the separation and the quantitative analysis of Dinotefuran . Azoxystrobin . Ipconazole 25% FS by HPLC with acetonitrile and water as mobile phase by gradient elution, using ZORBAX80A Extend - C18 column and DAD at 222nm wavelength was described. The result showed that the linear relative coefficient of 1. 000 0、1. 000 0、0. 999 9, the standard deviation of 0. 11、 0. 07、0. 01, the variation coefficient of 0. 59%、1. 24%、1. 38%, the average recovery of 99. 85%、100. 25%、100. 14% respectively, were obtained.