基于数据库分析肾透明细胞癌中DLG5的表达及预后判定作用

Utilizing databases to study the expression of DLG5 in clear cell renal cell carcinoma and its prognostic significance

目的通过研究DLG5(discs large homolog5)在肾透明细胞癌(ccRCC)临床组织中的表达,阐明其对ccRCC诊断与预后的作用。方法结合癌症基因组图谱(TCGA)数据库与基因表达综合(GEO)数据库、免疫组织化学法分析DLG5在ccRCC组织与正常肾组织中的表达差异,运用LinkedOmics、GEPIA、String-DB等分析DLG5表达与ccRCC临床病理指标、预后的关系及与其可能发生相互作用的蛋白网络。结果ccRCC中DLG5mRNA表达高于正常组织,LinkedOmics网站分析发现,其高表达患者总生存率低于低表达者(P=2.997×10-5);DLG5表达随TNM分期等级升高而增加;DLG5与增殖细胞核抗原(PCNA)表达的相关系数R=0.19(P=1.1×10-6),与RAD1表达的相关系数R=0.18(P=5.7×10-6),均呈正相关。结论DLG5高表达是ccRCC不良预后的指标,有望作为预测患者转移、复发、预后等的分子靶标。

Objective To clarify the role of discs large homolog 5 (DLG5) in the diagnosis and prognosis of renal clear cell carcinoma (ccRCC) by studying the expression of DLG5 in the clinical tissue of ccRCC. Methods DLG5 expression in ccRCC tissues and normal renal tissue was measured by using the Cancer Genome Atlas (TCGA) Database and Gene Expression Omnibus (GEO) Database. The correlation of DLG5 expression with the prognosis and clinicopathological parameters of ccRCC was analyzed by LinkedOmics and GEPIA. The interactions protein network of DLG5 was predicted by String-DB. Results Compared with that of the normal kidney tissues,DLG5 expression was significantly upregulated in the ccRCC tissues ( P <0.05). The high DLG5 expression was an independent prognostic factor affecting the overall survival rate of ccRCC based on TCGA ( P =2.997×10 -5 ). Furthermore,DLG5 expression had a positive correlation with the TNM stage,ethnicity,tumor purity,year-to-birth,PCNA ( P =1.1×10 -6 , R =0.19),and RAD1 ( P =5.7×10 -6 , R =0.18) expression of ccRCC. Conclusion In ccRCC,the high expression of DLG5 is a significant index of poor prognosis. Therefore,DLG5 can be a potential biomarker for predicting the metastasis,recurrence and prognosis of the patients.