摘要
目的检测长链非编码RNA(lncRNA)在鼻咽癌组织和正常鼻咽上皮组织中的差异表达,为研究lncRNA在鼻咽癌发生中的作用提供实验基础。方法分别选取3例鼻咽癌组织及3例正常鼻咽上皮组织,Trizol法提取总RNA,利用基因芯片技术,比较两组样本的lncRNA表达谱,筛选差异表达的lncRNA,并利用实时荧光定量PCR(qRT-PCR)方法进一步验证芯片结果。结果共筛选出454个差异表达的lncRNA,其中在鼻咽癌组织中上调表达173个,下调表达281个;与正常鼻咽上皮组织相比,lnc-RBBP8-1:2和lnc-MICALL2-1:8在鼻咽癌组织中均明显表达上调,差异有统计学意义(P<0.05),与芯片结果一致。结论与正常鼻咽上皮组织相比,鼻咽癌组织中存在明显的lncRNA差异表达。
Objective To detect the differential expression of long non-coding RNA (lncRNA) in nasopharyngeal carcinoma (NPC) tissues and normal nasopharyngeal epithelial tissues to provide experimental basis for studying the role of lncRNA in nasopharyngeal carcinogenesis. Methods Three cases of nasopharyngeal carcinoma tissues and three cases of normal nasopharyngeal epithelial tissues were selected. The total RNA was extracted with Trizol method. The lncRNA expression profiles of the two groups were compared by using gene chip technology. The differentially expressed lncRNAs were screened, and the chip results was further verified by using real-time fluorescence quantitative PCR (qRT-PCR). Results A total of 454 differentially expressed lncRNAs were screened, of which 173 were up-regulated and 281 were down-regulated in nasopharyngeal carcinoma tissues. Compared with that in normal nasopharyngeal epithelial tissues, the expressions of lnc- RBBP8-1:2 and lnc-MICALL2-1:8 in nasopharyngeal carcinoma tissues were all significantly higher, and the differences were statistically significant (P<0.05), which was consistent with the chip results. Conclusion Compared with normal nasopharyngeal epithelial tissues, there is a significant difference in the expression of lncRNA in nasopharyngeal carcinoma tissues.
作者
彭丽娇
李欣晓
江丹贤
张辉洁
陈竟
吴伟豪
黄静
PENG Li-jiao;LI Xin-xiao;JIANG Dan-xian;ZHANG Hui-jie;CHEN Jing;WU Wei-hao;HUANG Jing(Tumor Center,affiliated Hospital of Guangdong Medical University,Zhanjiang,Guangdong 524001;Department of Otolaryngology,affiliated Hospital of Guangdong Medical University,Zhanjiang,Guangdong 524001)
出处
《广东医科大学学报》
2019年第3期240-245,共6页
Journal of Guangdong Medical University
基金
广东省教育厅创新强校项目(No.2014KQNCX095)
湛江市财政科技专项竞争性分配项目(No.2017A01016)
关键词
鼻咽癌
长链非编码RNA
基因芯片
实时荧光定量PCR
nasopharyngeal carcinoma
long non-coding RNA
gene chip
real-time fluorescence quantitative PCR