摘要
Dear Editor, Artificial expression systems are crucial to functional genomics studies, constructing synthetic genetic circuits, and bioengineering (Werner & Gossen, 2014). They are usually achieved by cloning the coding sequence (CDS) of a target gene into a vector containing natural or engineered regulatory elements. However, this strategy is limited when target genes are hard to clone, for example when the CDS is too long, or expression level is too low.
基金
the National Natural Science Foundation of China (No.31530071)
Chongqing Research program of basic Research and Frontier Technology (No.cstc2017jcyjAX0349).