外源NO对康乃馨鲜切花保鲜效果的影响

Effect of Exogenous Nitric Oxide on Preservation of Fresh-cut Carnation

为研究外源NO对康乃馨鲜切花保鲜效果的影响,以康乃馨为试验材料,硝普钠(SNP)为外源NO供体,研究了不同浓度(0,50,100,150,200μmol·L^-1)SNP处理对康乃馨鲜切花瓶插寿命和花径大小的影响,以此筛选出最佳SNP处理浓度,并测定了最佳SNP处理浓度下,鲜切花花瓣中的可溶性蛋白含量、过氧化物酶(POD)和超氧化物歧化酶(SOD)活性的变化。结果表明:150μmol·L^-1的SNP处理康乃馨鲜切花瓶插寿命最长,比对照延长1.53d,延长率为11.57%;与对照相比,第8天花径提升率最高,为70.21%,第16天花径减退率最小,为23.61%。此外,150μmol·L^-1SNP处理明显提高了花瓣中的可溶性蛋白含量、POD和SOD活性,有效延缓了康乃馨鲜切花衰老。

In order to study the effect of exogenous NO on the preservation effect of carnation fresh cut flowers,effects of different concentrations of SNP (0,50,100,150,200 μmol ·L^-1 ) on the vase life and the flower diameter of cut carnation were investigated to choose the optimal SNP concentration,using SNP as a donor of exogenous NO.Meanwhile,The changes of soluble protein content,POD and SOD activity in fresh cut flower petals were determined by the optimal SNP concentration.The results showed that the longest vase life was recorded with 150 μmol ·L^-1 SNP treatment,which was 1.53 days longer than the control and the elongation rate was 11.57%.The highest flower diameter increasing rate (70.21%) was at 8 d and the lowest decreasing rate(23.61%) was at 16 d,compared to the control.In addition,soluble protein content,the activities of POD and SOD were significantly increased with 150 μmol ·L^-1 SNP treatment compared to the control.The senescence of fresh cut carnations was effectively delayed with 150 μmol ·L^-1 SNP treatment.