菱苡合剂对胃癌前病变大鼠前列腺素E2胃泌素、胃动素的影响

Effects of Lingyi Mixture （菱苡合剂） on Prostaglandin E2,Gastrin and Motilin in Rats with Gastric Precancerous Lesions

目的:观察菱苡合剂对胃癌前病变大鼠胃黏膜组织前列腺素E2(PGE2)、血清胃泌素(GT)、血浆胃动素(MTL)的影响。方法:采用自由饮用低浓度甲硝基亚硝胍(MNNG)液,联合乙醇灌胃的方法建立大鼠实验性胃癌前病变模型。模型成功后分别给予菱苡合剂、维酶素干预性治疗16周。实验结束后,检测并比较各组大鼠胃黏膜组织PGE2含量、血清GT、血浆MTL水平。结果:与正常组相比,模型组大鼠胃黏膜组织PGE2、血清GT均显著下降,血浆MTL水平升高,差异具有统计学意义(P <0. 01或P <0. 05);与模型组相比,菱苡合剂高、低剂量组、维酶素组的胃黏膜组织PGE2、血清GT均有不同程度升高(P <0. 01或P <0. 05);各给药组大鼠血浆MTL水平均有不同程度降低,其中维酶素组、菱苡合剂低剂量组与模型组相比,差异均具有显著性(P <0. 05)。菱苡合剂高剂量组大鼠血浆MTL水平较模型组虽有下降,但未达到统计学意义。结论:菱苡合剂能够调控胃癌前病变大鼠胃黏膜组织PGE2、血清GT、血浆MTL分泌水平,这可能是其防治胃癌前病变的作用机制之一。

Objective: To observe the effects of Lingyi Mixture on prostaglandin E2 (PGE2) gastrin (GT) and plasma motilin (MTL) in rats with gastric precancerous lesions. Methods: Setting up rat model of prophase pathological change of gastric carcinoma by freely drinking low concentration of MNNG solution and the absolute alcohol fills method. Lingyi Mixture or vitacoenzyme were given after the definition of success of copying models. At the end of the experiment, the levels of PGE2 in the gastric mucosa, serum GT and plasma MTL of each group were determined and compared. Results: Compared with the normal group, the gastric mucosal tissue PGE2 and serum GT decreased significantly in the model group, and the plasma MTL level increased, the difference was statistically significant (P <0. 01 or P <0. 05);Compared with the model group, Lingyi Mixture of high and low dose groups, vitacoenzyme group, the gastric mucosa PGE2 and serum GT of Lingyi Mixture high and low dose groups were increased,(P < 0. 01 or P < 0. 05);The MTL value of plasma in the rats of each medication group decreased in varying degrees, and the difference was significant (P < 0. 05) in Lingyi Mixture low group and vitamycin group compared with the model group. Lingyi Mixture group plasma MTL levels were lowered compared with the model group, but the difference was not statistically significant. Conclusion: Lingyi Mixture can regulate and control the gastric mucosal tissue PGE2 , serum GT and plasma MTL levels in rats with gastric precancerous lesions, which may be one of the main mechanism.