河南驻马店地区猪源肺炎克雷伯氏菌分离菌株生物学特性

Biological characteristics of a strain of Klebsiella pneumoniae isolated from pigs in Zhumadian

为了分析驻马店地区猪源肺炎克雷伯氏菌分离菌株生物学特性,从驻马店地区不同养殖场中采集患呼吸道疾病猪肺脏、鼻拭子等病料组织74份中分离得到了43株肺炎克雷伯氏菌。采用人工感染小鼠致病性试验、PCR方法和K-B药敏纸片分别检测49株肺炎克雷伯氏菌的致病性、血清型及耐药性。结果显示,30株肺炎克雷伯氏菌对小鼠具有很强的致病性;43株分离菌株以K5、K20为主要流行血清型,分别占分离菌株的37.2%、27.9%;43株分离菌株对氨苄西林、阿莫西林、大观霉素等7种药物耐药性较高,耐药率在51.2%以上,对其他药物的耐药率在18.6%~234.9%之间,且呈现多重耐药性,耐10、9种药物分离菌株最多,分别占分离菌株的25.6%、27.9%。本试验为该地区猪源肺炎克雷伯氏菌病的防治提供理论基础。

In order to analyze the biological characteristics of Klebsiella pneumoniae strains isolated from pigs in Sichuan area,43 strains of Klebsiella pneumoniae were isolated from pig lungs and nasal swabs collected from different farms in Sichuan.The pathogenicity,serotype and drug resistance of 49 strains of Klebsiella pneumoniae were analyzed by artificial infection in mice,PCR and K-B susceptible paper.The results showed that 30 strains of Klebsiella pneumoniae were highly pathogenic in mice,and 43 strains of K5 and K20 were the main epidemic serotypes,accounting for 37.2% and 27.9% of the isolated strains respectively.43 isolates showed high resistance to ampicillin,amoxicillin and spectinomycin.The resistance rate was over 51.2%.The resistance rate to other drugs ranged from 18.6% to 234.9%.The most resistant isolates were 10 and 9 kinds of drugs,accounting for 25.6% and 27.9% of the isolates,respectively.This study provides a theoretical basis for the prevention and control of Klebsiella pneumoniae in pigs.