广西地区艾滋病病毒感染/艾滋病患者蓝氏贾第鞭毛虫感染情况及基因型分析

Infection and genotype of Giardia lamblia among HIV/AIDS patients in Guangxi

目的了解广西地区艾滋病病毒感染/艾滋病(HIV/AIDS)患者蓝氏贾第鞭毛虫(简称贾第虫)感染情况及基因型分析。方法收集广西地区HIV/AIDS患者和健康者粪样,镜检后提取粪样中贾第虫DNA,并采用巢式PCR方法扩增贾第虫磷酸丙糖异构酶基因(triosephosphate isomerase, TPI),对阳性结果进行测序和BLAST比对分析,并构建进化树。结果共收集285例HIV/AIDS患者粪样,其中男性216例,女性69例,年龄21~85岁,其中130例曾接受抗逆转录病毒治疗。HIV/AIDS患者粪样镜检贾第虫阳性8份,阳性率为2.8%;健康者粪样303份,无贾第虫感染,两者差异有统计学意义(P <0.01)。巢式PCR扩增阳性粪样,发现530 bp左右有一条特异性条带。经测序分析,有7份贾第虫样品为人兽共患集聚体B型,其中6份与已报到的人源分离株TPI基因序列一致性为100%。另外1份为集聚体C型,且该分离株与已报到的人源贾第虫集聚体C型基因序列一致性为100%。结论广西地区部分HIV/AIDS患者感染贾第虫,其基因型主要为集聚体B型。

Objective To investigate the co-infection rate and the genotype of Giardia lamblia among HIV/ AIDS patients in Guangxi Autonomous Region. Methods Fecal specimens were collected from 285 HIV/AIDS patients and 303 HIV-negative people from Guangxi Autonomous Region. DNA were extracted from the feces and triosephosphate isomerase (TPI) gene of G. lamblia was amplified by nested PCR to determine the infection of G. lamblia. The amplified TPI PCR products were sequenced and the obtained sequences were aligned and analyzed by BLAST search. Results The overall co-infection rate of G. lamblia with HIV/AIDS was 2.8%(8/285). There was no G. lamblia infection detected in people without HIV-AIDS (P < 0.01). However, no significant risk factors were found to be related to the infection of G. lamblia among HIV-AIDS patients such as age, gender, sanitation, antiretroviral treatment or clinical manifestations (diarrhea, CD4+T lymphocyte level). Seven samples were identified to be zoonotic assemblage B and 6 had 100% sequence homology with the reported human-derived isolate. The other isolate was assemblage C and sequence identical to the reported human-derived isolate in our previous study. Conclusion HIV-AIDS patients have higher co-infection rate with G. lamblia than people without HIV infection. The major genotype of infected G. lamblia was assemblage B.