紫杉醇卡纳琳混悬液对胃癌细胞生长行为的的影响

Effect of paclitaxel carnaline suspension on growth behavior of gastric cancer

目的探讨紫杉醇卡纳琳混悬液对胃癌细胞增殖、侵袭的影响。方法利用免疫组化法检测Bcl-2,p16和p53的阳性表达。在HSC,SGC-7901以及NCI-N87三株胃癌细胞的培养液加入紫杉醇卡纳琳混悬液进行细胞增殖、细胞凋亡、细胞迁移和侵袭的体外实验。结果胃癌患者试验组及对照组淋巴细胞间Bcl-2,p16和p53阳性表达均无统计学差异(P>0.05);在体外,紫杉醇联合卡纳琳对3株胃癌细胞HSC,SGC-7901以及NCI-N87的凋亡具有促进作用,对细胞增殖、迁移和侵袭具有明显的抑制作用。结论紫杉醇联合卡纳琳混悬液后在体外细胞实验表明对胃癌细胞系的细胞增殖、细胞迁移和侵袭有明显抑制作用。

Objective To investigate the effects of taxol Carnarine suspension on the proliferation and invasion of gastric cancer cells. Methods The positive expression of Bcl-2, p16 and p53 was detected by immunohistochemical method. The proliferation, apoptosis, migration and invasion of HSC, SGC-7901 and NCI-N87 gastric cancer cells were studied by adding paclitaxel Carnarine suspension in vitro. Results There was no significant difference in the expression of Bcl-2,p16 and p53 between the experimental group and the control group (P>0.05). Paclitaxel combined with Carnarine can promote the apoptosis of HSC, SGC-7901 and NCI-N87 in three gastric cancer cells, and inhibit the proliferation, migration and invasion of three gastric cancer cells. Conclusion In vitro cell experiments with paclitaxel combined with carnaline suspension showed significant inhibition of cell proliferation,cell migration and invasion of gastric cancer cell lines.