一测多评法测定转移因子口服液中核糖核酸的含量

RNA quantitative detection method studying for transfer factor oral solution by QMSA

目的:建立转移因子口服液中核糖核酸的一测多评含量测定方法。方法:对转移因子口服液酶解条件、色谱方法筛选,以鸟嘌呤脱氧核苷酸为内参物,建立其余核苷酸,核苷及总核糖核酸含量的一测多评法。结果:各核苷酸及核苷峰形好,分离度高,一测多评法及多对照外标法测定结果基本一致。结论:此方法实现了转移因子口服液中核糖核酸的准确定量,同时提高检测效率,降低检测成本,适用于转移因子口服液中的核糖核酸的检测与监控。

Objective:To establish a quantitative analysis of multi-components by singer-marker (QMSA) assay method for ribonucleic acid of transfer factor oral solution(TFO). Methods:Through the enzymatic hydrolysis condition of TFO and the HPLC method optimization, a QMSA assay method was established for nucleotide, nucleoside and total ribonucleic acid by using the guanine deoxynucleotide as the internal reference. Results:the peak shape of each nucleotide and nucleoside were good and the resolution was high. The result of QMSA method and multi-control external standard method were basically the same. Conclusion:the method realizes accurate quantification of ribonucleic acid in TFO, improves the detection efficiency, reduces the detection cost. It is suitable for the detection and monitoring ribonucleic acid in TFO.