HPLC-ELSD测定艾附暖宫丸中黄芪甲苷的含量

Content Determination of Astragaloside IV in Aifu Nuangong Pills by HPLC-ELSD

目的建立艾附暖宫丸中黄芪甲苷的含量测定方法,为其提供质量标准。方法应用高效液相色谱蒸发光散射检测器,LanboSMTC18100A色谱柱(250mm×4.6mm,5μm),以乙腈-水(32∶68)为流动相,流速为1.0mL·min^-1,ELSD参数:雾化室温度36℃,漂移管温度60℃,增益值130,载气压力30psi。结果黄芪甲苷在15.67~109.69μg·mL^-1范围内呈良好线性关系,r=0.9994(n=6),平均回收率为98.69%。结论该方法分离度高,重现性好,灵敏度高,无干扰,可用于艾附暖宫丸的质量控制。

OBJECTIVE To establish a method for the content determination o f astragaloside IV in aifu nuangong pills. METHODS HPLC-ELSD d etector was adopted.The chromatography was carried out on Lanbo SMT C 18 100A column (250mm×4.6mm,5μ) with acetonitrile-water (32∶ 68) as the mob ile phase at a flow rate of 1.0mL·min^ -1 ,ELD parameter:atomizing temper ature was 36℃ and the drift tube temperature was maintained at 60℃.The gain v alue was 130 and the pressure of nebulizing gas was 30psi. RESULTS The linear range of astragaloside Ⅳ was 15.67~109.69μg·mL ^-1 ,r=0 .9994(n=6).The average recovery was 98.69%. CONCLUSION The m ethod is high separation efficiency,repeatability,sensibility and no interfere nce.It can be utilized as a quality control method for Aifu Nuanging Pills.