滋阴明目丸对RCS大鼠视网膜Fas/FasL表达的影响

Effects of Ziyin Mingmu Pills on the expression of Fas/FasL in the retina of RCS rats

目的观察滋阴明目丸对RCS大鼠视网膜Fas/FasL表达的影响。方法选择RCS大鼠,(1.51±0.27)月龄,共24只,按随机数字表法将其分为三组:空白组、模型组、滋阴明目丸组(雌雄各4只,n=8)。空白组:RCS(rdy+/+,p+/+)大鼠,灌胃生理盐水;模型组:RCS(rdy-/-,p-/-)大鼠,灌胃生理盐水;滋阴明目丸组:RCS(rdy-/-,p-/-)大鼠,灌胃滋阴明目丸。灌胃30 d后,免疫组化染色观察标本视网膜各层结构的形态学变化,Western blot法测定RCS大鼠视网膜Fas/FasL表达水平。结果免疫组化染色结果显示:空白组大鼠视网膜各层结构清晰;模型组大鼠视网膜的厚度低于空白组,且各层结构不清晰;滋阴明目丸组大鼠视网膜厚度低于空白组但高于模型组,视网膜各层结构较清晰。Western blot法检测结果显示:滋阴明目丸组大鼠视网膜Fas、FasL蛋白相对表达量明显低于模型组,差异有高度统计学意义(P <0.01)。结论滋阴明目丸对视网膜的超微结构具有保护作用,能抑制视网膜上Fas/FasL的表达,从而减轻视网膜感光细胞的凋亡,达到保护视细胞的目的。

Objective To observe the effect of Ziyin Mingmu Pills on the expression of Fas/FasL in the retina of RCS rats. Methods The RCS rats were selected, aged (1.51±0.27) months, total 24 rats, they were randomly divided into 3 groups: blank group, model group, Ziyin Mingmu Pills group (4 male and 4 female, n = 8). Blank group: RCS (rdy+/+, p+/+) rats were given normal saline by intragastric administration;model group: RCS (rdy-/-, p-/-) rats were given normal saline by intragastric administration;Ziyin Mingmu Pills group: RCS (rdy-/-, p-/-) rats were given Ziyin Mingmu Pills by intragastric administration. After intragastric administration for 30 d, the morphological changes of each layer of the retina sample were observed by immunohistochemistry. The expression levels of Fas/FasL in the retina of rats were determined by Western blot. Results The results of immunohistochemistry showed that each layer of retinal structure of the rats in the blank group was clear;the thickness of retina in the model group was lower than that in the blank group and the structure of each layer of retina was not clear;the retinal thickness of Ziyin Mingmu Pills group was lower than that of blank group but higher than that of blank group, the structure of each layer of retina was clear. The results of Western blot assay showed that the relative expression of Fas, FasL protein in the retina of Ziyin Mingmu Pills group was lower than that in the model group, the difference was highly statistically significant (P < 0.01). Conclusion Ziyin Mingmu Pills has a protective effect on the ultrastructure of retina, which can inhibit the expression of Fas/FasL of the retina and reduce the apoptosis of retinal photoreceptor cells, thus achieving the purpose of protecting visual cells.