转化生长因子β对人肾小管上皮细胞全基因表达谱的影响及其生物信息学分析

Effect of transforming growth factor-β (TGF-β) on gene expression profile of human tubular epithelial cells and bioinformatic analysis

目的本研究探讨转化生长因子-β(TGF-β)对人肾小管上皮细胞(HK-2)基因表达谱的影响及相关生物信息学分析,为深入探讨和研究TGF-β对肾功能的影响及作用机制提供理论依据和实验基础。方法将体外培养的HK-2细胞随机分为正常对照组和TGF-β诱导组,培养48h后取出,运用Illumina测序平台检测TGF-β对HK-2细胞基因表达谱的影响,并采用DAVID数据库对差异表达的基因(mRNA)进行其富集的GO功能和基于KEGG数据库的生物通路富集分析。实时定量RT-PCR用于验证基因表达谱结果。结果TGF-β处理HK-2细胞48h后,基因测序分析筛选出表达差异>1.5倍的基因共279个,其中上调121个,下调158个,包括钙离子结合、脂质代谢、细胞黏附、血管钙化、炎症反应等相关的基因。RT-PCR验证结果基因测序分析结果一致。结论TGF-β可能通过多条通路调控肾小管上皮的功能。

Objective The aim of this study was to explore the effect of the transforming growth factor-beta (TGF-β) on gene expression profile of human tubular epithelial cells (HK-2) and perform related bioinformatics analysis, so as to provide theoretical and experimental basis for further investigation of the effect of TGF-β on renal functions and its mechanism. Methods HK-2 cells cultured in vitro were randomly divided into normal control group and TGF-β-induced group. After culturing for 48 h, the Illumina sequencing platform was used to test the effect of TGF-β on the gene expression in HK-2 cells, and the DAVID database was applied to examine differentially expressed genes (mRNA) for its concentrated GO function and biological pathway concentration analysis based on KEGG database. Real-time quantitative RT-PCR was used to verify the results of the gene expressing profile. Results After HK-2 cells were treated with TGF-β for 48 h, a total of 279 genes, including 121 up-regulated genes and 158 down-regulated ones, which are associated with calcium ion binding, lipid metabolism, cellular adhesion, vascular calcification and inflammatory reactions. The result of RT-PCR was consistent with those of gene sequencing analysis. Conclusions TGF-β probably regulate the function of renal tubular epithelial cells through multiple pathways.