实时荧光定量聚合酶链反应快速检测MRSA的应用研究

Evaluation of the application of real-time fluorescence quantitative PCR for rapid detection of MRSA

目的研究实时荧光定量聚合酶链反应(PCR)检测耐甲氧西林金黄色葡萄球菌(MRSA)的临床应用。方法收集2017年1月至2018年12月重庆市南川区人民医院患者体液标本分离培养的446例金黄色葡萄球菌,采用实时荧光定量PCR检测金黄色葡萄球菌nuc基因和mecA基因。结果446例金黄色葡萄球菌均检出nuc基因,符合率为100.00%。经全自动细菌培养鉴定出的96例MRSA中均检出mecA基因,检出符合率为100.00%。在全自动细菌培养鉴定及药敏分析仪上检出的甲氧西林敏感金黄色葡萄球菌中有2例检测到mecA基因,其检测正确率为97.96%(96/98)。结论实时荧光定量PCR检测MRSA耗时短,正确率高,用于临床快速鉴定MRSA有明显优势。

Objective To study the clinical application of real-time fluorescent quantitative PCR for detection of methicillin-resistant Staphylococcus aureus (MRSA). Methods A total of 446 cases of Staphylococcus aureus were collected from body fluid samples from patients in Nanchuan District People′s Hospital of Chongqing from January 2017 to December 2018.The nuc gene and mecA gene of Staphylococcus aureus were detected by real-time fluorescence quantitative PCR. Results Nuc gene was detected in 446 cases of Staphylococcus aureus,and the coincidence rate was 100.00%.MecA gene were detected in a total of 96 cases which were ideatified,as MRSA by fully automatic bacterial culture,and the coincidence rate was 100.00 %.MecA gene was detected in 2 cases of MRSA which were detected by automatic bacterial culture identification and drug sensitivity analyzer,and the detection accuracy was 97.96%(96/98). Conclusion Real-time fluorescence quantitative PCR detection of MRSA is time-consuming and accurate,which has obvious advantages in rapid clinical identification of MRSA.