重组大肠杆菌top10(PET28a-makinase9)抗逆性分析

Stress Tolerance Analysis of Escherichia Coli Top10 Expressing the Eukaryote Transcription Initiation Factor makinase9

将来源于蓝藻的Makinase9基因导入载体PET28a中,在原核表达的基础上,利用分光光度计检测重组大肠杆菌top10(PET28a-makinase9)的抗逆性。重组大肠杆菌top10(PET28a-makinase9)的抗NaCl在一定浓度范围内会随盐浓度增大而增强,对抗NaHCO3较为敏感,表现为一定范围内既有促进又有抑制,不具有抗O2^2-和抗Cu^2+胁迫性。为蓝藻的二元系统和双组分系统在其他生物渗透胁迫信号识别和转导中的研究提供了依据。

To get the fundamental function of the Eukaryote transcription initiation factor makinase9, this experiment introduced the Makinase9 gene of Cyanobacteria into the vector PET28a for the purpose of prokaryotic expression and then detect the stress resistance of E. colire combinant top10 (PET28a-makinase9) with spectrophotometer. The result showed the stress resistance for NaCl in E. colirecombinant top10 (PET28a-makinase9) increa ses when the NaCl concentration grew by some range and the stress resistance for NaHCO3 was sensitive expressed as promotion and inhibitionin some range of concentration, but had not obvious resistant toO2^2- and Cu^2+ stress. E. coli top10 expressing the Makinase9 gene had obviousre sistance to salt and alkali stress and had not resistance to O2^2- and Cu^2+ stress. These results of binary system and Two-Component systems in Cyano bacteria provide scientific basis for the research of signal recognition and transduction in other bio-infiltration.