超薄射流和低温等离子体协同膜组合系统对枯草芽孢杆菌沾染物的洗消效能研究

Decontamination efficacy of combined system of ultrathin jet stream,low temperature plasma and collaborative membrane on Bacillus subtilis

目的 检验新型生物洗消技术——超薄射流和低温等离子体协同膜组合系统(代号UJS-LTP-WSD-1)对枯草芽孢杆菌(模拟炭疽芽胞杆菌)沾染物的节水洗消效能,为应对生物恐怖袭击及灾害提供有效去除高危病原微生物沾染的新手段。方法 采用UJS-LTP-WSD-1系统(UJS组),以普通喷淋系统(普通组)作对照,对在一定浓度枯草芽孢杆菌悬液中浸泡过的覆漆钢板、生猪皮和背部脱毛雄性成年家兔,分别冲洗10s、20s、30s、40s、60s、90s,然后取样进行细菌培养,对检出的菌落数进行比较。根据UJS-LTP-WSD-1喷头水流速和单位时间内菌落检出数的差异,推算节水率。结果 对于枯草芽孢杆菌沾染的覆漆钢板、生猪皮和背部脱毛成年雄兔,UJS组和普通组的洗消效果显示随时间延长枯草芽孢杆菌的检出数均显著降低,但UJS组的洗消效果显著优于普通组(P<0.05)。UJS模块洗消枯草芽孢杆菌沾染覆漆钢板、生猪皮、背部脱毛雄性成年家兔3组的节水率分别为85.2%~90.5%、91.5%和81.5%。结论 UJS-LTP-WSD-1系统是一种可对芽孢杆菌类细菌进行高效节水洗消的集成系统,有望成为应对生物恐怖袭击或灾害的新手段。

Objective To examine the efficacy of a novel biological decontamination system (ultrathin jet stream, low temperature plasma and collaborative membrane, Code UJS-LTP-WSD-1) on water-saving decontamination of Bacillus subtilis, which was used to mimic the contamination of Bacillus anthracis, and to provide new approach for effective elimination of high-risk pathogenic microorganism contaminants pertaining to bioterrorism attacks and major disasters. Methods A UJS-LTP-WSD-1 (UJS) system and a conventional spray system (as control) were used to rinse paint-coated steel plates, raw pig skins and back-depilated adult male rabbits, which were previously immersed in Bacillus subtilis suspension at certain concentration. The rinse durations were 10 s, 20 s, 30 s, 40 s, 60 s and 90 s. After rinse, sampling was performed for bacterial culture, the number of detected colony forming unit (CFU) was compared. The water-saving rates (WSRs) were analyzed according to the flow velocities of UJS-LTP-WSD-1 sprayer and the number of detected CFU in unit time. Results For the Bacillus subtilis contaminated paint-coated steel plates, raw pig skins and back-depilated adult male rabbits, the number of detected Bacillus subtilis in UJS and control group significantly decreased along with time, the contamination efficacy in UJS was significantly better than that in control group (P < 0.05). The calculated WSRs were 85.2%-90.5%, 91.5% and 81.5% for paint-coated steel plates, raw pig skins and back-depilated adult male rabbits, respectively. Conclusions UJS-LTPWSD- 1 system is a high-efficient integrated system for water-saving decontamination of Bacillus pathogenic microorganisms, and is a promising approach against biological attacks or disasters.