抑癌基因PTEN参与多柔比星非霍奇金淋巴瘤Raji细胞生长、迁移侵袭的实验研究

Effects of PTEN Combined with Adriamycin on the Proliferation,Migration and Invasion of Adriamycin in Non Hodgkin lymphoma Raji Cells

目的研究多柔比星对非霍奇金淋巴瘤细胞株Raji体外增殖、迁移侵袭的作用及PTEN(gene of phosphate and tension homology deleted on chromsome ten)的影响。方法四甲基偶氮唑蓝(MTT)法测定不同浓度多柔比星对Raji细胞生长活力的影响;Transwell法检测多柔比星和PTEN对Raji细胞迁移侵袭的影响;RT-qPCR检测多柔比星对Raji细胞中PTENM表达的影响。结果多柔比星具有显著抑制Raji细胞增殖的作用,且呈浓度依赖效应(r=-0.925,P<0.001);多柔比星能够抑制Raji细胞的侵袭和迁移;多柔比星能够促进PTEN在Raji细胞中的表达,过表达PTEN能显著抑制Raji细胞的侵袭和迁移;多柔比星和PTEN联合使用能够显著降低Raji细胞活力、抑制Raji细胞侵袭和迁移能力。结论多柔比星和PTEN均能够抑制Raji细胞增殖、侵袭和迁移,PTEN可显著增强多柔比星对Raji细胞增殖、迁移侵袭的抑制作用。

OBJECTIVE To investigate the inhibitiom effect of PTEN(gene of phosphate and tension homology deleted on chromsome ten)combined with adriamycin on proliferation,migration and invasion of non-Hodgkin lymphoma cell line Raji in vitro.METHODS Cell proliferation was determined by MTT in Raji cells response to adriamycin with different concentrations.Transwell assay was performed to evaluate the effects of adriamycin and PTEN on migration and invasion of Raji cells.RT-qPCR was conducted to measure the expression of PTEN in Raji cells after adriamycin treatment.RESULTS Adriamycin significantly inhibited the proliferation of Raji cells in a concentration dependent manner(r=-0.925,P<0.001).Adriamycin inhibited invasion and migration in Raji cells.Moreover,adriamycin promoted the expression of PTEN.Overexpression of PTEN markedly suppressed invasion and migration in Raji cells.The combination of adriamycin and PTEN strikingly decreased the proliferation,invasion and migration of Raji cells.CONCLUSION Adriamycin and PTEN would inhibite the proliferation,invasion and migration of Raji cells.PTEN drastically enhances the inhibition of adriamycin on the proliferation,migration and invasion of Raji cells.