T-淋巴细胞亚群在患儿血液病诊断中的应用研究

Application of T-lymphocyte Subsets in Diagnosis of Children with Hematological Diseases

目的:研究T-淋巴细胞亚群在血液病诊断的临床价值。方法:选取2016年3月-2018年6月在笔者所在医院就诊的60例血液病患儿为研究对象,作为观察组。同时选取60例健康患儿作为对照组。所有参与试验者均采用流式细胞仪检测外周T-淋巴细胞亚群。对比两组的检测结果、不同类型血液病患儿外周T-淋巴细胞亚群与对照组间的差异性,以及血液病患儿治疗前后外周T-淋巴细胞亚群的变化情况。结果:观察组CD3^+、CD4^+及CD4^+/CD8^+等指标的水平均明显低于对照组,CD8^+水平明显高于对照组,差异均有统计学意义(P<0.05)。相比对照组,观察组淋巴瘤、多发性骨髓瘤、慢性白血病与急性白血病患儿的CD3^+、CD4^+及CD4^+/CD8^+水平均较低,CD8^+水平均较高,差异均有统计学意义(P<0.05)。治疗后,观察组CD3^+、CD4^+及CD4^+/CD8^+等指标水平显著升高,CD8^+水平显著降低,差异均有统计学意义(P<0.05)。结论:检测血液病患儿的T-淋巴细胞亚群能够有效帮助临床诊断,值得进一步研究并推广应用。

Objective:To study the clinical value of T-lymphocyte subsets in the diagnosis of children with hematological diseases.Method:Sixty children with hematological diseases who were treated in our hospital from March 2016 to June 2018 were selected as the observation group.At the same time,60 healthy children were selected as the control group.All of the participating investigators used flow cytometry to detect peripheral T-lymphocyte subsets.The results of the two groups were compared and analyzed.The differences between peripheral T-lymphocyte subsets and control groups in different types of hematological patients were compared and analyzed.In addition,compared the peripheral T-lymphocyte subsets before and after treatment in patients with hematological diseases. Result:The levels of CD3^+,CD4^+ and CD4^+/CD8^+ in the observation group were significantly lower than those in the control group,and the level of CD8^+ was significantly higher than that of the control group,the differences were statistically significant(P<0.05).Compared with the control group,the levels of CD3^+,CD4^+ and CD4^+/CD8^+ in lymphoma,multiple myeloma,chronic leukemia and acute leukemia in the observation group were lower,and the level of CD8^+ was higher, the differences were statistically significant(P<0.05).After treatment,the levels of CD3^+,CD4^+ and CD4^+/CD8^+ in the observation group increased significantly, while the level of CD8^+ decreased significantly,the differences were statistically significant(P<0.05).Conclusion:The detection of T-lymphocyte subsets in children with hematological diseases can effectively help clinical diagnosis,which deserves further research and popularization.