烟草拮抗链霉菌FT05W基因组测序与几丁质酶家族基因鉴定

Genome Sequencing and Identification of Chitinase Family Genes of Streptomyces sp. FT05W,an Antagonist of Tobacco Pathogens

链霉菌FT05W是一株对烟草黑胫病等土传真菌病害具有良好拮抗作用的生防放线菌。本研究利用Miseq PE300高通量测序平台对链霉菌FT05W基因组进行序列测定,共获得6914188个reads,通过SPAdes软件对序列进行拼接共得1836个contigs,其基因组全长为7699129 bp,GC比为71%,其中编码基因序列长度为6037181 bp,预测出7434个蛋白编码基因(GenBank登录号:NZ_QGMR00000000),预测编码7323个蛋白。获得的基因注释信息为今后深入开展其在烟草上的生物防治机理研究奠定了重要的基础,有助于推动烟草生防链霉菌功能基因的挖掘与利用,为烟草生防链霉菌FT05W的进一步开发与应用提供理论依据。利用MEGA 6.06构建链霉菌FT05W的几丁质酶家族基因系统发育树,共鉴定出7个几丁质酶家族基因,其中6个为18家族几丁质酶基因,1个为19家族基因。

Streptomyces sp.FT05 W was a biocontrol strain with antagonism to tobacco soil-borne fungal pathogens.In this study,we used Miseq PE300 next-generation sequencing platform to perform the genome sequencing,and obtained 6914188 reads.The SPAdes software was used to splice a total of 1836 contigs with a total genome length of 7699129 bp and a GC ratio of 71%.Length of the coding gene sequence was 6037181 bp,and 7434 protein-coding genes were predicted,which are predicted to encode 7233 proteins.Subsequently,through the comparison and analysis of databases such as GO,COG,KEGG,NR and TCDB,the genome prediction and basic function annotations were completed.The obtained gene annotation information lays an important foundation for further research on its biological control mechanism in tobacco.The results will help to promote the excavation and utilization of Streptomyces sp.functional genes and provide a theoretical basis for further development and application of Streptomyces sp.FT05 W.Finally,the chitinase family gene phylogenetic tree of Streptomyces sp.FT05 W was constructed using MEGA 6.06.A total of seven chitinase gene families were identified.Six were family18 chitinase genes and one was family 19 gene.