高、低糖甘蔗品种伸长期糖分积累特征及代谢相关酶活性分析

Analysis of sugar accumulation characteristics and metabolism-relateed enzymes activities in the high and low sugar sugarcane at elongation stage

为了探究高、低糖甘蔗品种伸长期蔗糖代谢相关酶与糖分积累的相关性,采用桶栽试验,对甘蔗高糖(GT35)和低糖(B8)品种伸长期不同成熟度的叶和茎中的蔗糖合成方向和分解方向酶的酶活性、蔗糖、葡萄糖和果糖含量进行分析。结果表明,2个品种叶中蔗糖和己糖含量与NI酶活性呈显著正相关,茎中蔗糖含量与SPS和CIN酶活性呈显著正相关,与SS-c、SAI和NI酶活性呈显著负相关。GT35在成熟茎和老茎中蔗糖含量显著高于B8,己糖含量则显著低于B8。GT35中酶活性显著高于低糖B8的酶有成熟叶中的SPS、SS-c和SAI,老叶中的SPS、SS-c、NI和CIN,幼叶中的SS-s和NI,成熟茎中的SPS和CIN,老茎中的SPS、SS-s和CIN以及幼茎中的SS-c和CIN。B8中酶活性显著高于GT35的酶有幼叶中的SS-c、SAI和CIN,成熟叶中的SAI和CIN,老叶中的SAI和SS-s,幼茎中的SAI、SS-s和NI,成熟茎中的SS-s、SS-c和SAI,老茎中的NI。基于以上结果,认为叶中高SPS和NI酶活性,茎中SPS和CIN酶活性高,SAI、SS-c和NI酶活性低,可能是调节伸长期高糖甘蔗品种茎中蔗糖积累的重要因素。

To analyze the correlation between sucrose metabolism-related enzymes and sugar accumulation in high and low sugar sugarcane,the experiment was conducted with the split plot to analyze the sucrose synthesis and decomposition direction enzyme activity,sucrose,glucose and fructose content in leaves and stems with different maturity at the elongation stage of high sugar(GT35)and low sugar(B8)sugarcane.The results showed that the contents of sucrose and hexose in the leaves of GT35 and B8 were positively correlated with the activity of neutral/alkaline invertase(NI),the sucrose content in stem was positively correlated with sucrose phosphate synthase(SPS)and cell wall-bound invertase(CIN)activity,and negatively correlated with sucrose synthetase in the cleavage direction(SS-c),soluble acid invertase(SAI)and NI activity.The sucrose content in maturing and matured stems of GT35 was significantly higher than that of B8,while the content of hexose was significantly lower than that of B8.The enzyme activity in high-sugar GT35 was significantly higher than that in low-sugar B8,including SPS,SS-c and SAI in maturing leaves,SPS,SS-c,NI and CIN in matured leaves,sucrose synthetase in the synthesis direction(SS-s)and NI in immature leaves,SPS and CIN in maturing stems,SPS,SS-s and CIN in matured stems,and SS-c and CIN in immature stems.The enzyme activity in low-sugar B8 was significantly higher than that in high-sugar GT35,including SS-c,SAI and CIN in immature leaves,SAI and CIN in maturing leaves,SAI and SS-s in matured leaves,and SAI,SS-s and NI in immature stems,SS-s,SS-c and SAI in maturing stems,and NI in matured stems.The analysis results demonstrate that high SPS and NI activities in leaves,high SPS and CIN activities in stems,and low SAI,SS-c and NI activities in stems may be important factors for regulating sucrose accumulation in high-sugar varieties of sugarcane at elongation stage.