摘要
相较于气相色谱-质谱(GC-MS)和高效液相色谱(HPLC),衰减全反射傅里叶变换红外光谱(ATR-FTIR)能实现绘画颜料中蛋白质种类的“不分离即分析”,从而简化分析步骤和减小文物样品的消耗。这项研究验证了ATR-FTIR用于颜料中蛋白质种类鉴别的可行性。以石青和白垩为矿物颜料,牛奶、明胶和鸡蛋为蛋白质粘合剂制备了新鲜样品和经紫外光老化的模拟文物样品,运用二阶导数光谱和主成分分析建立了模式识别模型。结果表明模拟文物样品中蛋白质的种类能通过新鲜样品建立的模型进行识别。因此,ATR-FTIR在文化遗产领域中蛋白质种类的微损和快速鉴别具有巨大的潜力。
Compared with gas chromatography-mass spectroscopy(GC-MS) and high performance liquid chromatography(HPLC), attenuated total reflection Fourier transform infrared(ATR-FTIR) spectroscopy is an "analysis-without-separation" technique so that the analytical procedures can be simplified and the consumption of the art samples can be minimized in the analysis of color layers in paintings. This study examined the feasibility of the ATR-FTIR spectroscopy for identifying the protein binders in color layers. The fresh and accelerated UV light aged reference samples with single protein binders and the mixtures of pigment-protein binders were prepared, by using milk, animal glue, egg white as protein binders, and chalk, azurite as pigments. Second derivative infrared(SD-IR) spectral pattern recognition models were obtained by using principal component analysis(PCA). The protein binders in artificial art samples can be discriminated by the pattern recognition models, which were established based on the analysis of fresh samples. Therefore, the ATR-FTIR spectroscopy has great potential in micro-destructive and rapid chemical identification of protein binders in the field of cultural heritage.
作者
许昆
王菊琳
XU Kun;WANG Ju-lin(Key Laboratory of Electrochemical Process and Technology for Materials,Beijing University of Chemical Technology,Beijing 100029,China;School of Materials Science and Engineering,Beijing University of Chemical Technology,Beijing 100029,China;Key Research Base of State Administration of Cultural Heritage for Evaluation of Science and Technologyin Cultural Relics Protection Field,Beijing 100029,China)
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2019年第7期2299-2306,共8页
Spectroscopy and Spectral Analysis
基金
National Natural Science Foundation of China(51471020)
关键词
ATR-FTIR
主成分分析
紫外加速老化
蛋白质粘合剂
文化遗产保护
ATR-FTIR spectroscopy
Principal component analysis
Accelerated UV aging process
Protein binders
Cultural heritage conservation