2012-2018年安徽省风疹病毒基因特征分析

Genetic characterization of rubella virus circulated in Anhui province, 2012-2018

目的分析2012-2018年安徽省流行风疹病毒(Rubella virus,RV)的基因特征。方法从安徽省14个地市获得2012-2018年RV分离株,扩增和测定其基因序列(nt8731-9469),鉴定RV基因型,分析与WHO参考株、中国其他省份流行株、其他国家和地区流行株的基因亲缘性关系和序列差异。结果获得安徽省2012-2018年RV分离株127株,经鉴定,1E基因型21株、2B基因型106株。1E基因型RV毒株流行于2012-2013年,属于中国本土流行的1E-ClusterA优势基因亚型,2014-2018年未检测到该基因型,2018年检测到输入型1E基因型毒株。2012-2018年多数2B基因型毒株与中国其他省份同期流行的2B基因型毒株一致,同属于2B-ClusterC基因亚型,2012年同时检测到输入型2B基因型毒株。安徽省流行的1E和2B基因型RV在E1基因的重要功能区域均高度保守。结论 2012-2018年安徽省流行RV基因型发生了新的变化,呈现多样性特点。

Objective To analyze the genetic characterization of rubella virus (RV) strains circulating in Anhui province during 2012-2018.Methods We obtained RV strains from 14 prefectures of Anhui during 2012-2018 to amplify and sequence a target gene (nt8731-9469) and determined RV genotypes. We analyzed phylogenetic trees and sequence variations compared with WHO reference strains, strains from other Chinese provinces, and strains from other countries or regions. Results In total, 127 RV strains were obtained in Anhui during 2012-2018;21 and 106 strains were identified as 1E and 2B genotypes, respectively. The 1E genotype strain circulated in 2012-2013, and belonged to the indigenous Chinese 1E-ClusterA subgenotype;this genotype was not found during 2014-2018, however an imported genotype 1E strain was detected in 2018. Most genotype 2B strains during 2012-2018 belonged to the 2B-ClusterC subgenotype and were the same as those isolated from other Chinese provinces. An imported 2B genotype strain was also detected in 2012. Both 1E and 2B genotype strains viruses that circulated in Anhui were highly conserved in the important functional region of the E1 gene. Conclusions The genotypes of RVs circulating in Anhui changed during 2012-2018 and showed diverse characteristics.