网脉橐吾提取物镇痛、抗炎活性研究

Study on Analgesic and Anti-inflammatory Activity of Extract from Ligularia dictyoneura (Franch.) Hand.-Mazz

目的研究网脉橐吾Ligularia dictyoneura(Franch.)Hand.-Mazz提取物的镇痛、抗炎活性,并对其安全性进行初步评价。方法采用小鼠单次灌胃给药急性毒性试验,观察受试物对小鼠的急性毒性反应和死亡情况;采用醋酸、热板、甲醛所致小鼠疼痛模型,评价受试物的镇痛活性;采用二甲苯致小鼠耳肿模型,初步评价受试物的抗炎活性。结果网脉橐吾提取物小鼠灌胃急性毒性试验的LD50为3.65 g/kg,95%可信限为3.30~4.04 g/kg。给药后动物出现毒性反应,主要表现为俯卧、运动减少、运动失调、腹泻、抽搐、呼吸困难等症状。镇痛试验结果显示,与溶媒对照组比较,网脉橐吾提取物可明显抑制醋酸、热板、甲醛所致小鼠疼痛反应。抗炎实验结果表明,与溶媒对照组比较,网脉橐吾提取物对二甲苯所致小鼠耳肿胀度有明显抑制作用。结论网脉橐吾提取物对改善炎症及疼痛具有一定作用。

Objective To study the analgesic,anti-inflammatory activity and safety of Extract from Ligularia dictyoneura(Franch.)Hand.-Mazz(ELD).Methods The acute toxicity reaction and mortality in mice were evaluated by acute toxicity assay.Pain models caused by acetic acid,hot stimulation and formaldehyde were used to observe the analgesic activity of ELD.Mouse ear swelling model induced by xylene was used to measure the anti-inflammatory activity of ELD.Results The LD50 of ELD was 3.65 g/kg and the 95%confidence limit was 3.30 g/kg-4.04 g/kg.After the administration,animals showed some toxic reactions,such as:pronate,exercise reduction,movement disorders,diarrhea,tremor,breath with difficulty.Compared with the vehicle group,the ELD group could significantly inhibit the pain response of mice induced by acetic acid,hot stimulation and formaldehyde.Moreover,the ELD group could markedly inhibit mice ear swelling caused by xylene.Conclusion The Extract of Ligularia dictyoneura(Franch.)Hand.-Mazz showed a certain effect on inflammation and pain.