基于胶原蛋白的干细胞3D打印研究进展

3D Bioprinting with Stem Cells Based on Collagen

干细胞3D打印是以“生物打印机”为手段,以干细胞、生长因子、生物材料等为主要内容重建人体组织或器官的跨学科领域的新型再生医学工程技术,有着美好的发展前景和巨大的社会价值。但细胞活性的维持、打印材料和打印过程与细胞的相容性、快速凝胶化等要求限制了可打印水凝胶材料的选择。胶原蛋白是天然生物组织中细胞外基质的重要成分,优异的生物相容性使其在干细胞3D打印中有天然优势。综述了基于胶原蛋白的几种物理、离子作用及共价作用凝胶化方式,以及干细胞3D打印支架在组织工程上的研究进展。

The 3D bioprinting with stem cells has great prospect in organ or tissue repair. It constructs the 3D scaffolds with stem cells,growth factors and hydrogel biomaterials to regenerate the injured organs with the help of 3D bioprinter. However,the printable hydrogel biomaterials are deficient by considering the maintained activity of stem cells,biocompatibility between cells and the materials and the printing process,fast gelation property and so on. Collagen,an essential component of extracellular matrix in natural tissues,has emerged as a hydrogel material for 3D printing with great advantages in encapsulating stem cells. In this review,the gelation of collagen hydrogels in physical,ion-bonding and covalent-bonding ways and their advances in 3D bioprinting with stem cells for tissue engineering are highlighted.