HLA-B27基因亚型与汉族人群强直性脊柱炎遗传易感性的研究

Analysis of susceptibility gene subtypes of HLA-B27 in ankylosing spondylitis population

目的探讨汉族人群人类白细胞抗原B27(HLA‐B27)基因亚型在强直性脊柱炎(AS)的表达及亚型分析,评价其检测在临床上的应用价值。方法选取2016年1~12月南昌大学第二附属医院门诊及住院AS患者74例,其中,类风湿关节炎(RA)65例,腰椎盘间盘突出(LIDH)51例,脊柱结核(ST)50例,骨关节炎(OA)53例。选取40例作为健康对照,应用BDFACSCalibur流式细胞仪检测细胞HLA-B27抗原;聚合酶链反应-直接测序分型(PCR-SBT)技术对HLA-B27基因亚型进行分型。结果AS患者HLA-B27阳性率高于RA、LIDH和ST疾病及健康对照组(P<0.007);30~<40岁年龄组男性人群HLA-B27阳性率高于女性人群(P<0.05);AS患者HLA-B27基因亚型以B2704为主。结论南昌汉族人群中AS患者HLA-B27基因亚型以B2704、B2705为主,且B2704是AS的发病易感基因亚型。

Objective To analyze gene susceptible subtype of AS in Nanchang.Methods Totally 74 cases of AS,65 cases of RA,51 cases of LIDH,50 cases of ST,53 cases of OA and 40 healthy people who were admitted into the Second Affiliated Hospital of Nanchang University during January 2016 to December 2016 were involved in this study.HLA-B27 antigen were detected by BD FACScalibur flow cytometry.Sub-Genotyping of HLA-B27 gene were achieved by polymerase chain reaction direct sequencing typing(PCR-SBT)technique.Results We found that positive rate HLA-B27 was increased in patients with AS compared with that in RA,LIDH,ST,OA and healthy controls(P<0.05).The positive rate of HLA-B27 was higher in men than in women aging from 30 to 40(P<0.05).B2704 is the major gene subtype of HLA-B27 in AS patients(P<0.05).Conclusions Major subtypes of HLA-B27 gene in AS patients is B2704 and B2705 which is related to the incidence of AS in Han population in NanChang.