双低油菜籽蛋白氨基酸组成分析及营养价值评价

Amino acid composition and nutritional evaluation of double low rapeseed proteins

以4种双低油菜籽为原料,使用氨基酸自动分析仪测定脱脂菜籽粕中菜籽蛋白的氨基酸组成,并应用氨基酸比值系数法和模糊识别法对其营养价值进行评价。结果表明,4种双低菜籽蛋白中均可检测到19种氨基酸;4种菜籽蛋白的总氨基酸含量在809.99~855.11 mg/g蛋白,必需氨基酸占氨基酸总量的34.4%~35.5%,必需氨基酸与非必需氨基酸含量之比为0.524~0.543;4种双低菜籽蛋白的氨基酸比值系数为87.71~90.96,庆油3号菜籽蛋白的第一限制氨基酸为异亮氨酸,其他品种菜籽蛋白的第一限制氨基酸均为蛋氨酸+胱氨酸;4种双低菜籽蛋白与FAO/WHO模式的贴近度在0.959~0.975,与鸡蛋蛋白模式贴近度在0.872~0.894。该研究为菜籽蛋白产品开发提供理论依据,也为双低油菜育种提供更全面的视角。

Four kinds of double low rapeseed were selected as raw materials.The amino acid composition of proteins from defatted rapeseed meals were determined by amino acid automatic analyzer,and their nutritional values were evaluated by SRCAA and fuzzy recognition method.The results showed that there were 19 amino acids detected in all four double low rapeseed proteins.The total amino acids contents were 809.99-855.11 mg/g protein,with essential amino acids accounted for 34.4%-35.5%.Moreover,the ratios of essential amino acids to non-essential amino acids were 0.524-0.543,and the scores of SRCAA were 87.71-90.96.It was found that the first limiting amino acid in Qingyou No.3 rapeseed protein was isoleucine,and others were limited in methionine and cystine.Furthermore,the close degrees of the four double low rapeseed proteins to the standard protein(FAO/WHO)ranged from 0.959 to 0.975,and to egg proteins were 0.872-0.894.This study provides a theoretical basis for developing rapeseed protein products as well as a more comprehensive perspective for double low rapeseed breeding.