盐胁迫下盐穗木miR393b与预测靶基因Hc TIR1的表达及相关性

Expression and Correlation Analysis of the Halophyte Halostachys caspica miR393b with Predictive Target Gene HcTIR1 under Salt Stress

【目的】研究盐胁迫下盐穗木生长素通路中miR393b和预测的靶基因 TIR1 的表达模式和相关性。【方法】采用生物信息学方法预测盐穗木miR393b的靶基因;通过qRT-PCR方法检测盐胁迫下盐穗木miR393b及预测靶基因 HcTIR1 ( transport inhibitor response1 )的相对表达模式,并分析其相关性。【结果】 miR393b成熟体在不同植物种中高度保守;盐穗木miR393b预测的靶基因为 TIR1;盐胁迫处理,盐穗木同化枝中两基因响应高盐胁迫,并具有一定的负相关性。【结论】盐穗木miR393b和预测的靶基因响应高盐胁迫,二者具有一定的负相关。该结果为进一步探讨盐穗木miR393b与预测靶基因 TIR1 的生物学功能奠定基础。

【Objective】 microRNA , as a kind of small endogenous non-coding RNA, affects plant various biological process by negatively regulating the expression of corresponding target genes. Halostachys caspica as a salt-diluted short shrub belonging to the Chenopodiaceae is distributed widely in acrid and saline-alkali region of Xinjiang and shows extreme salt-tolerance. In this study, the expression patterns and correlations of miR393b and the predicted target gene TIR1 of this species under salt stress were discussed.【Method】 The analysis of bioinformatics was used to predict the target gene using the H. caspica transcriptome data. The relative expression of Halostachys caspica miR393b and TIR1 was detected by qRT-PCR under salt stress using this species' branches and the correlation analysis was performed.【Result】 miR393b between different plant species is highly conserved through sequence alignment;The H.caspica branches miR393b and HcTIR1 responded to salt stress and showed a certain negative correlation under high sanility of 600 mM NaCl.【Conclusion】 Halostachys caspica miR393b and the predictive target gene responded to salt stress and both of them had a certain negative correlation under high salt concentration. The results of this study laid the foundation for further exploration of the biological function of miR393b and HcTIR1 in the halophyte Halostachys caspica.