花生幼苗期呼吸酶活性对渍涝的响应

Responds of Respiration Related Enzymes Activity to Waterlogging Stress Occurred at Germination and Seedling Stages of Peanut

渍涝是我国南方地区花生芽苗期频发的灾害,其造成的直接效应是缺氧,改变植物的呼吸途径。本研究选取3个经大田验证具有明显耐涝差异的花生品种,在幼苗期设置不同时间、不同深度渍涝胁迫处理,测定3种呼吸关键酶琥珀酸脱氢酶(SDH)、乙醇脱氢酶(ADH)、乳酸脱氢酶(LDH)的活性变化,以明确不同品种对湿涝胁迫的呼吸适应机制。结果表明,耐涝性强的品种表现出SDH活性高、LDH和ADH活性低,而耐涝性弱的品种SDH活性低、LDH和ADH活性高,该结果与大田耐涝性鉴定结果一致。

Waterlogging frequently occurs at germination and seedling stages of peanut in southern China, which leading to the lack of oxygen and change of respiratory pathway of plants. Three peanut cultivars with significantly different tolerance to waterlogging, which had been identified in the field, were chosen as materials in this study. The activity variations of three key respiratory enzymes, succinate dehydrogenase (SDH), alcohol dehydrogenase (ADH) and lactate dehydrogenase (LDH), of the seedlings were measured to clarify the respiratory adaptation mechanisms of the three peanut varieties to waterlogging under the treatment of different time and different depths of waterlogging stress. The results showed that the waterlogging-resistant cultivars presented high SDH activity, low LDH and ADH activities, while the sensitive cultivars had low SDH activity,high LDH and ADH activities. The results were consistent with the evaluation results of field waterlogging tolerance.